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Culture of Jerusalem Artichoke in vitro and Assessment of Genetic Stability Using SSR(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年20
Page:
91-94
Research Field:
Publishing date:

Info

Title:
Culture of Jerusalem Artichoke in vitro and Assessment of Genetic Stability Using SSR
Author(s):
YANG ShipengZHAO MengliangSUN XuemeiTAO LitingLI Li
(Qinghai Vegetable Genetics and Physiology Laboratory,College of Agriculture and Forestry Sciences,Qinghai University,Xining,Qinghai 810016)
Keywords:
jerusalem artichoketissue cultureSSRPCRgenetic stability
PACS:
-
DOI:
10.11937/bfyy.201520022
Abstract:
Taking ‘QingYu 2’ stem segment explants as materials,the effect of different media on jerusalem artichoke callus induction,shoot induction and rooting culture was studied.The genetic stability of subculture jerusalem artichoke plantlets with different generation were analyzed using optimized SSRPCR reaction system.The results showed that suitable media for cultivating jerusalem artichoke plants in vitro was: MS+1.00mg/L 6BA+0.50 mg/L NAA,and 1/2MS+0.10 mg/L IBA was suitable for rooting.Analysis of genetic variation from the subculture 8 times in tissue culture,no variation between individuals among the 50 detected bands,also no significant differences in the biological traits.The results indicated that genetic material was not significantly changed in jerusalem artichoke,and provided a technology platform for propagation of jerusalem artichoke in future.

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Last Update: 2015-11-19