|Table of Contents|

Establishment of Two-dimensional Electrophoresis System of Grape Seed(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年20
Page:
86-91
Research Field:
Publishing date:

Info

Title:
Establishment of Two-dimensional Electrophoresis System of Grape Seed
Author(s):
WANG Ruipu1; CUI Lei1; ZHANG Xiaoying2; WANG Qian2; ZHANG Chaohong2; LI Yan1
（1.College of Life Sciences，Northwest Agriculture and Forest University,Yangling,Shaanxi 712100；2.College of Horticulture，Northwest Agriculture and Forest University，Yangling，Shaanxi 712100）
Keywords:
grape; seed; proteomics; Two-dimensional electrophoresis (2-DE)
PACS:
-
DOI:
10.11937/bfyy.201520021
Abstract:
Taking the grape seeds as material，in order to establish a two-dimensional electrophoresis（2-DE）system for proteomic analysis of grape seed，we compared the parameters including different protein extraction methods，the pH value gradient of IPG strip，sample loading，isoelectric focusing conditions and different SDS-PAGE separation gel.The results showed that the protein was dissolved quickly，more pure and in high concentration after extracting by TCA-acetone/phenol method.The system with 17 cm pH 5-8 IPG strips，loading 600 μg samples，focusing to 80 000 Vh and seperating by 11% SDS-PAGE gel showed good two-dimensional electrophoresis maps.The system was also suitable for different grape seed protein.

References:

 

[1]PANDEY A，MANN M.Proteomics to study genes and genomics [J].Nature，2000，405(6788)：837-846.

[2]GUARNIERI M T.Comparative proteomics lends insight into genotype-specific pathogenicity[J].Proteomics，2013，13(17):2544-2545.

[3]陶然，王晨，房经贵，等.我国葡萄育种研究概况[J].江西农业学报，2012，24（6）：24-30.

[4]GIRIBALDI M,PERUGINI I,SAUVAGE F X,et al.Analysis of protein changes during grape berry ripening by 2-DE and MALDI-TOF [J].Proteomics，2007，7(17)：3154-3170.

[5]VINCENT D,WHEATLEY M D,CRAMER G R.Optimization of protein extraction and solubilization for mature grape berry clusters[J].Electrophoresis，2006，27(9)：1853-1865.

[6]焦竹青，许培磊，王振兴，等.山葡萄花序蛋白质双向电泳技术体系的建立[J].果树学报，2012，29(5)：945-951.

[7]陈蕊红，张改生，刘卫，等.小麦花药蛋白质组双向电泳技术体系的优化[J].核农学报，2008，22(4)：404-409.

[8]巩红飞，柳军玺，邸多隆.蛋白质组学研究中的分离分析技术及其研究进展[J].氨基酸和生物资源，2014，36(2)：12-17.

[9]GAO M X，LI N，ZHANG J,et al.The study of three extraction methods for preseparation and enrichment：application to the complex proteome separation in rat liver[J].Separation and Purification Technology，2006，52(1)：170-176.

[10]GORG A,WEISS W,DUNN M J.Current two-dimensional electrophoresis technology for proteomics [J].Proteomics，2005(3)：826-827.

[11]李德军，邓治，陈春柳，等.植物组织双向电泳样品制备方法研究进展[J].中国农学通报，2009，25(24)：78-82.

[12]刘伟霞，潘映红.适用于小麦叶片蛋白质组分析的样品制备方法[J].中国农业科学，2007，40(10)：2169-2176.

[13]马斌，孙骏威，余初浪，等.枇杷叶片和果实总蛋白质提取及双向电泳的优化方法[J].果树学报，2011，28(2)：358-362.

[14]SARRY J E,SOMMERER N,SAUVAGE F X,et al.Grape berry biochemistry revisited upon proteomic analysis of the mesocarp[J].Proteomics，2004，4(1)：201-215.

Memo

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Last Update: 2015-11-19