|Table of Contents|

Optimization of SSR Analysis System in Eggplant(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年17
Page:
70-74
Research Field:
Publishing date:

Info

Title:
Optimization of SSR Analysis System in Eggplant
Author(s):
LI Ye
(Harbin Academy of Agricultural Science，Harbin,Heilongjiang 150070)
Keywords:
eggplant; SSR; analysis system
PACS:
-
DOI:
10.11937/bfyy.201517018
Abstract:
Taking eggplant DNA of ‘Hanongzaqie No.4’ as the test material，using single factor test method，the SSR amplified results of eggplant and comparative analysis of polymorphism of SSR detected on two gel electrophoresis systems were studied.A standard SSR system fitting for eggplant was established.The results showed that the optimized amplification conditions were 20-40 ng DNA，0.3 μmol/L of each primer，200 μmol/L of dNTP，2.0-2.5 mmol/L Mg2+ and 1 uint Taq DNA polymerse in 20 μL volume.The optimized PCR cycles started at 94℃(5 min)，followed by 35 cycles of 1 min at 94℃，1 min at 52-56℃ and 2 min at 72℃，and then extended 8 min at 72℃.6% Polyacrylamide gel had higher ability than 1.8% agarose gel in discriminating polymorphism.

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Last Update: 2015-10-10