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RAPD Analysis on Three Cucurbita ficifotia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年17
Page:
75-78
Research Field:
Publishing date:

Info

Title:
RAPD Analysis on Three Cucurbita ficifotia
Author(s):
CHENG Xiaojing1; ZHOU Chuqi1; ZHOU Liying1; BU Lulu1; HONG Jiankang2; YANG Zhengan1
(1.College of Horticulture,Yunnan Agricultural University,Kunming,Yunnan 6502101;2.Honghe Prefecture Agricultural Institute of Yunnan Province,Mengzi,Yunnan 661100）
Keywords:
Cucurbita ficifotia Bauche; genome DNA; RAPD; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.201517019
Abstract:
The genomic DNA of 3 Cucurbita ficifotia whose names were ‘Green peel’,‘White peel’ and ‘Green peel with White peel’ respectively were extracted by CTAB and were used to analysis the genetic relationships by RAPD molecular markers.RAPD was affected by many factors，so this experiment was for improved RAPD method.5 factors and 4 levels orthogonal design and screening of RAPD-PCR reaction system was used，the three kinds of black seed pumpkin relatedness were analyzed.The results showed that from 38 kinds of random primers selected 20 kinds of primers，6 kinds of the primers’analysis was clear amplification，and better repeatability.Total 43 bands were amplified，in which 39 bands were polymorphic．The percentage of polymorphic band was 90.70%．The cluster analysis results were indicated as follows：the average genetic distance among ‘Green peel’，‘White peel’ and ‘Green peel with white spot’ was 0.94.The genetic distance between ‘White peel’ and ‘green peel with white spot’ was 0.40.The results showed that the Cucurbita ficifotia varieties of relationships：‘Green peel’ and ‘White peel’，‘Green peel with white spot’ had far more distant relatives，and then‘White peel’ and ‘Green peel with white spot’ had close genetic relationship.

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Last Update: 2015-10-10