Pathogen Identification and Biological Characteristics of Rose Leaf Blotch in Tianjin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Pathogen Identification and Biological Characteristics of Rose Leaf Blotch in Tianjin

Author(s):: FENG Youren; LIU Baosheng; BAI Penghua; (Tianjin Institute of Plant Protection，Tianjin 300381)

Keywords:: Rosa chinensis Jacq; leaf blotch; pathogen identification; biological characteristics; Pestalotiopsis clavispora (Arth.)Stey.

Abstract:: Taking one disease on leaf of Rosa chinersis that found in Tianjin flower nursery as test material，the pathogen was identified through morphological characteristics，pathogenicity and rDNA-ITS sequence analysis，simple biological characteristics research of this pathogen was conducted also.The results showed that fungal colony that developed on PDA was 34.6-37.2 mm in diameter，with concentric rings in white in four days.The conidia was fusiform and 4.83-9.11 μm in diameter，formed by five cells with four transverses；the apical appendage of conidia was 2-3，5.93-36.23 μm in length；the basal appendage of conidia was 1，2.85-16.05 μm in length.The homology of rDNA-ITS sequence between this pathogen and Pestalotiopsis clavispora was 99%.Based on the morphological characteristics and rDNA-ITS sequence analysis，the pathogen was finally identified as P.clavispora.This was the first report that Pestalotiopsis clavispora could cause leaf blotch disease of Rosa chinensis Jacq in China.Biological characteristics studies demonstrated that the optimum temperature for growth was 25℃，no significant difference existed in the range of 20-30℃；the suitable pH value was around 5.0；the suitable carbon source was lactose.

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Last Update: 2015-08-14