|Table of Contents|

Extraction and Determination of Apple Leaf Total Flavonoids(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年14
Page:
129-132
Research Field:
Publishing date:

Info

Title:
Extraction and Determination of Apple Leaf Total Flavonoids
Author(s):
Korbanjhon?BRAD1; WANG Meng2; ZHANG Xiaoying1; 2
(1.College of Chemistry and Biological Sciences，Yili Normal University，Yining，Xinjiang 835000;2.College of Veterinary Medicine，Northwest Agriculture and Forestry University，Yangling，Shaanxi 712100)
Keywords:
apple leaves; total flavonoids; extraction method; concentration measurement
PACS:
-
DOI:
10.11937/bfyy.201514034
Abstract:
Taking dry apple leaf of ‘Red delicious’ as test material，the optimized extraction method for the apple leaves was explored by the single factor experiment.The total flavonoids were quantitative determined by visible spectrophotometer,to supply an optimized method and methodological basis for flavonoids extraction from apple leave.The results showed that the optimum extraction conditions of apple leaves flavonoids were diluted in 60% ethanol by the ratio of 1∶20 g/mL，70℃ for 30 minutes.Under the above conditions，the flavonoids concentration in apple leaves was measured and took rutin as standard.The linear regression was good within the range of 4-24 μg of rutin and the equation was Y＝24.821 43X-0.031，R2＝0.999 45 (n=6).The total flavonoid in apple leaves was 30.28 mg/g at average.And had set up an optimized flavonoid extraction and assessment methods for apple leave.This method is simple，rapid，accurate，precision，stable and good repeatable.

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Last Update: 2015-08-14