|Table of Contents|

Identification of SSR in 75 Cultivated Grape Varieties(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
115-119
Research Field:
Publishing date:

Info

Title:
Identification of SSR in 75 Cultivated Grape Varieties
Author(s):
LI XueyanLIANG HaiyongXIAN LijieYANG Minsheng
Key Lab of Genetic Resources of Forest and Forest Protection of Hebei Province,Hebei Agricultural University,Baoding,Hebei 071000
Keywords:
grapesimple sequence repeat makerscluster analysis
PACS:
S 663.102.4
DOI:
10.11937/bfyy.201513033
Abstract:
In this experiment,75 parts of grape cultivars were selected as test materials,using SSR molecular markers to study the establishment of a suitable grape DNA fingerprinting technology to build library system for the identification and genetic relationship grape germplasm resources,to provide the basis for analysis.The results showed that the choice of 19 pairs of core primers were amplified 89 polymorphic loci by DPS software,its cluster analysis,75 parts of the grape germplasm genetic similarity coefficient 0.028 3-0.887 3 average genetic similarity coefficient was 0.475 39.According to use,initially divided into table grape and wine grape into two categories,depending on the variety and origin,divided into the European and American hybrids,American hybrids and Eurasian species.

References:

[1]刘鑫铭.葡萄种质表型遗传多样性分析及初级核心种质构建[D].洛阳:河南科技大学,2010.[2]张立平.葡萄属RAPD分类研究[J].园艺学报,1998,25(2):191-194.[3]张立平,林伯年,沈德绪,等.葡萄属植物核糖体基因的RFLP分析[J].园艺学报,1997,24(4):385-387.[4]方连玉,王军,许雷.15份葡萄种质遗传多样性的SSR分析[J].分子植物育种,2010,8(3):511-515.[5]温景辉,申海林,邹利人,等.20份葡萄种质亲缘关系的SSR分析[J].果树学报,2011,28(5):782-786.[6]蔡斌,李成慧,姚泉洪,等.葡萄全基因组SSR分析和数据库构建[J].南京农业大学学报,2009,32(4):28-32.[7]Adam Blondon A F,Roux C,Claux D,et al.Mapping 245 SSR markers on the Vitis vinifera genome:a tool for grape genetics[J].Theor Appl Genet,2004,109(5):1017-1027.[8]Thomas M R,Scott N S.Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as sequence tagged sites(STSs)[J].Theor Appl Genet,1993,86(6):985-990.[9]姚玉新,左方梅,翟衡,等.‘蛇龙珠’等酿酒葡萄品种的DNA分析[J].园艺学报,2005,32(4):604-608.[10]Scott K D,Eggle P,Seatox G,et al.Analysis of SSRs derived from grape ESTs[J].Theoretical and Applied Genetics,2000,100(5):723-726.

Memo

Memo:
-
Last Update: 2015-08-21