|Table of Contents|

Establishment of Wild Lilium Tissue Culture System(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年11
Page:
95-97
Research Field:
Publishing date:

Info

Title:
Establishment of Wild Lilium Tissue Culture System
Author(s):
ZHANG Yue; WANG Jian-li; WANG Xiu-feng; CHENG Ying-kui; MA Yi-qiao; YU Yong-hui; LIU Jing-li
Jilin Research Institute of Vegetables and Flowers,Changchun,Jilin 130033
Keywords:
Lilium pumilum; organogenesis; somatic embryogenesis
PACS:
S682.2+65
DOI:
10.11937/bfyy.201511023
Abstract:
Taking  Lilium pumilum as experimental material,using tissue culture method,effects of different parts of Lilium pumilum, different hormone combinations and different ways of plant regeneration on Lilium pumilum were studied.The results showed that with the bulb as explants,the organ regeneration plants were obtained,the best medium for differentiation and proliferation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L；in flower organ,the young leaves as explants via somatic embryo genesis regeneration plants were obtained,and the callus induction medium was MS+2,4-D 2.0 mg/L+NAA 1.5 mg/L，adventitious bud induction medium was MS+6-BA 1.0 mg/L+IAA 0.1 mg/L，the optimal proliferation medium was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.Rooting culture medium was 1/2MS+NAA 0.1 mg/L.

References:

[1] 葛蓓孛，杨青杰，吴萍，等．细叶百合组织培养植株再生[J]．东北林业大学学报,２０１０,３８(５):５４-５９.[2] 吴若著,阮少宁.百合胚状体形成途径及植株的再生研究[J].福建林学院学报,2003,23(4):322-325.[3] 万勇,毛凌华,黄永萍,等.万载百合组织培养快速繁殖的研究[J].江西农业学报,2000,12(4):26-29.[4] 唐东芹,黄丹枫,唐克轩,等.东方百合鳞片的组织培养[J].植物生理学通讯,2003,39(5):450-452.[5] 王元忠,沙本才.南川百合的组织培养和快速繁殖[J].亚热带植物科学,2008,37(3):69-70.[6] 张艺萍,屈云慧,吴学尉,等.大理百合的组织培养和快速繁殖[J].北方园艺,2007(8):189-190.[7] 周祖富,艾素云,杨美纯.火百合花丝组织培养器官形成的细胞组织学研究[J].亚热带植物科学,2006,41(3):1-15.[8] 刘菊华,金志强,徐碧玉,等.龙牙百合的植株再生与遗传转化[J].分子植物育种,2003，4(1):465-474.

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Last Update: 2015-08-06