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Establishment of Dendrobium officinale Mycorrhiza System and the Study on Its Related Gene Expression(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年11
Page:
98-102
Research Field:
Publishing date:

Info

Title:
Establishment of Dendrobium officinale Mycorrhiza System and the Study on Its Related Gene Expression
Author(s):
MAO Pei-peiZHOU Li-tingWU LinWU Jian-bingWANG Hui-zhong
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants,College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,Zhejiang 310036
Keywords:
Dendrobium officinaleMycena dendrobiisymbiosisdifferential expression
PACS:
S 682.31
DOI:
10.11937/bfyy.201511024
Abstract:
The study focused on the interaction mechanisms between mycorrhizal fungi and host plants.Taking Mycena dendrobii and Dendrobium officinale as materials,the symbiotic system of M.dendrobii and D.officinale was constructed.The microscopic observation of microsection of symbiont showed that M.dendrobii-D.officinale mycorrhizal symbiont was established successfully.Simultaneously,the chorismate synthase gene and mannose-specific lectin gene were tested using fluorescent quantitative Polymerase Chain Reaction,showing that these genes were up-regulated in early symbiosis stage.The results showed that these genes participate in the regulation of symbiosis.This study laid the foundation for clarifying the interaction molecular mechanisms between orchid and mycorrhizal fungi.

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Last Update: 2015-08-06