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The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年11
Page:
90-94
Research Field:
Publishing date:

Info

Title:
The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6
Author(s):
WANG Chong; CHENG Yu-xiang
State Key Laboratory of Tree Genetics and Breeding，Northeast Forestry University，Harbin,Heilongjiang 150040
Keywords:
Arabidopsis thaliana; GDPD-Like; expression pattern; subcellular localization
PACS:
Q 945
DOI:
10.11937/bfyy.201511022
Abstract:
Using wild type (Col-0) Arabidopsis thaliana as plant materials，the expression pattern of AtGDPDL6 and AtGDPDL7 was studied by RT-PCR.Using the ProAtGDPDL6::GUS and ProAtGDPDL7::GUS transgenic plants as plant materials，the expression pattern of AtGDPDL6 and AtGDPDL7 promoter were studied by GUS activity staining.Using the Pro35S::AtGDPDL6-GFP transgenic plants as plant materials，the subcellular localization of GDPDL6 was studied by fluorescence microscopy.The results showed that AtGDPDL6 was specifically expressed in flowers，and AtGDPDL7 was expressed in flowers and siliques;ProAtGDPDL6::GUS and ProAtGDPD7::GUS were mainly expressed in mature pollen sacs and stigma;the AtGDPDL6 was localized to the plasma membrane or the cell wall.

References:

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Last Update: 2015-08-06