|Table of Contents|

Estimation of Genome Size of Cymbidium faberi Rolfe(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年11
Page:
86-90
Research Field:
Publishing date:

Info

Title:
Estimation of Genome Size of Cymbidium faberi Rolfe
Author(s):
WANG Chen-ying1; 2; ZHANG Kun3; LIANG Fang4; CUI Bo4; MA Run-lin5
1.College of Life Science，Zhengzhou Normal University，Zhengzhou，Henan 450044；2.Molecular Biology Laboratory，Zhengzhou Normal University，Zhengzhou,Henan 450044；3.College of Life Science，Heilongjiang University，Harbin，Heilongjiang 150080；4.Institute of Bioengineering，Zhengzhou Normal University，Zhengzhou,Henan 450044；5.Institute of Genetics and Development Biology,Chinese Academy of Sciences,Beijing 100101
Keywords:
Cymbidium faberi; genome size; flow cytometry; Dendrobium officinal
PACS:
S 682.31
DOI:
10.11937/bfyy.201511021
Abstract:
aking Cymbidium faberi as material，using D.of ficinale Kimura et Migo as an external reference,flow cytometry measurements were performed when the genome size of Cymbidium faberi was estimated.The results showed that,the estimated genome size of C.faberi was (4.39±0.12）Gb,or the content of 2C DNA was about 8.98 pg.The present study described a modified method of genome size analysis by using flow cytometry in C.faberi.The data available of DNA content obtained in this study was helpful to the future genome sequencing research.

References:

[1] Doleel J,BartoJ.Plant DNA flow cytometry and estimation of nuclear genome size[J].Ann Bot,2005,95(1):99-110.[2] 桂毅杰,王晟,金艳丽，等.毛竹基因组大小和序列构成的比较分析[J].中国科学C辑(生命科学）,2007,37(4):488-492.[3] 孙永星,李素花,魏小丽,等.适合流式细胞仪分析的小麦细胞核提取方法比较[J].生物学杂志,2012,29(3):88-91.[4] 李蔚,刘莉莎,李仁,等.十字花科蔬菜基因组含量的测定与分析[J].植物遗传资源学报,2011,12(1):103-106.[5] 吴丽萍,唐岩,李颖岳,等.枣和酸枣基因组大小的测定[J].北京林业大学学报,2013,35(3):77-83.[6] 张志珂,王永清,林顺权,等.借助流式细胞仪进行枇杷基因组学测序材料的倍性分析[J].果树学报,2012,29(3):498-504.[7] 邓果特,刘清波,蒋建雄,等.五节芒基因组大小的测定[J].植物遗传资源学报,2013,14(2):339-341.[8] Galbraith D W，Harkins K R，Maddox J R，et al.Rapid flow cytometric analysis of the cell cycle in intact plant tissues[J].Science,1983,220:1049-1051. [9] Pfosser M，Amon A，Lelley T，et al.Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines [J].Cytometry,1995,21(4)：387-393.[10]Dolezel J，Binarová P，Lucretti S.Analysis of nuclear DNA content in plant cells by flow cytometry[J].Biol Plant,1989,31(2):113-120.[11]Loureiro J，Rodriguez E，Dolezel J，et al.Two new nuclear isolation buffers for plant DNA flow cytometry：A test with 37 species[J].Ann Bot,2007,100(4):875-888.[12]黎燕萍.云南首绘铁皮石斛全基因组图谱解生命密码拓产业先机[N].大公报,2013-09-20.[13]Dolezel J，Barto J，Voglmayr H，et al.Nuclear DNA content and genome size of trout and human [J].Cytometry A,2003,51(2):127-128.[14]贾少强,卢博林.兰花基因组框架图在深完成[N].深圳商报,2009-11-16.[15]田云芳,蒋素华,袁秀云，等.蕙兰GAPDH基因的全长克隆及生物信息学分析[J].江西农业学报,2013,35(4):870-877.[16]李小玲,赵娜,华智锐.商洛山区野生花资源及其生境土壤特性调查[J].河南农业科学,2011,40(1):112-114.[17]Johnston J S，Bennett M D，Rayburn A L，et al.Reference standards for determination of DNA content of plant nuclei[J].Am J Bot,1999,86(5):609-613. [18]Bennett M D,Leitch I J,Price H J,et al.Comparison of Caenorhabditis (100 Mb) and Drosophila (175 Mb) using flow cytometry show genome size in Arabidopsis to be (157 Mb) and thus 25% larger than the Arabidopsis Genome Initiative estimate of 125 Mb[J].Ann Bot,2003，91:547-557.[19]弓娜,田新民,周香艳，等.流式细胞术在植物学研究中的应用-检测植物核DNA含量和倍性水平[J].中国农业通报,2011,27(9):21-27.

Memo

Memo:
-
Last Update: 2015-08-06