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Study on Inducing Hairy Roots of Solanum nigrum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
107-111
Research Field:
Publishing date:

Info

Title:
Study on Inducing Hairy Roots of Solanum nigrum L.
Author(s):
WANG Li; LIU Qi; NING Ming-ming; ZHANG Wei-dong; QI Xiao-li; LI Xiu-xia
(College of Life Science，Jiamusi University，Jiamusi,Heilongjiang 154007)
Keywords:
Solanum nigrum; Agrobacterium rhizogenes; hairy roots inducement; condition optimization
PACS:
-
DOI:
10.11937/bfyy.201504025
Abstract:
Agrobacterium rhizogenes strains of A4，C58C1，A1476 were selected to discuss effect of explants types，Agrobacterium rhizogenes species，infection time，pre-culture time，concentration of bacterium，co-cultivation period on hairy roots inductivity of Solanum nigrum.The results showed that with leaf as explants，the inductivity was the highest.Agrobacterium rhizogenes strains of A4，C58C1，A1476 could all induce hairy roots，but inductivity of C58C1 was higher.Better infection time was 5 minutes.The inductivity at OD600=0.6 was better performing.The inductivity was higher when the time of pre-culture and co-cultivation was 2 days.It provided experimental basis for the mass production of hairy roots and alkaloid of Solanum nigrum production which used hairy root culture techniques.

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Last Update: 2015-04-20