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Study on the Mutagenic Effects of Space Environment on Cyclamen persicum Mill.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
112-115
Research Field:
Publishing date:

Info

Title:
Study on the Mutagenic Effects of Space Environment on Cyclamen persicum Mill.
Author(s):
LI Jin1GENG Jin-peng1CAO Tian-guang1WEI Wen-bin2ZHENG Zhi-xing2ZHAN Yong1
(1.School of Sciences,Hebei University of Technology,Tianjin 300401;2.Institute of Garden Flowers,Zhangjiakou Academy of Agricultural Sciences,Zhangjiakou,Hebei 075000)
Keywords:
space environmentCyclamen persicum Mill.phenotypic traitsmutantsrandom amplified polymorphic DNA analysis
PACS:
-
DOI:
10.11937/bfyy.201504026
Abstract:
To explore the mutagenic effect of space environment on Cyclamen persicum Mill.,dry seeds were carried by Chinese“Shen Zhou VIII”.After recovery,these seeds were planted and investigated.The results showed the first generation of the space-exposed plants displayed considerable changes of phenotypic traits in flower color and flower shape compared with their control plants.Mutated plants which acquired new phenotypic traits after space flight were analyzed by random amplified polymorphic DNA (RAPD) analysis.Among 28 random primers used in this experiment,27 primers generated different DNA band type.156 DNA bands were produced,among which 91 DNA bands were polymorphic with the percentage of polymorphism being 58.33%.The DNA of mutated plants had changed,and the DNA mutation rates varied from 23.08% to 33.97%,which suggested that dramatic changes on DNA molecular level occurred after the plants had been exposed to the space environment.Results of the study demonstrated that the mutagenic effects could occur in the seeds flown in space,and the space environment could make changes in some of the genes,and these changes in the genes would induce phenotypical changes.

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Last Update: 2015-04-21