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Comparative Research of Total RNA Extraction Methods From Apocynum venetum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年11期
Page:
93-96
Research Field:
Publishing date:

Info

Title:
Comparative Research of Total RNA Extraction Methods From Apocynum venetum L.
Author(s):
LI Miao1; 2; LI Guo-qi1
1.Key Lab for Restoration and Recovery of Degraded Ecosystem in Northwestern of Ministry of Education,Ningxia University,Yinchuan,Ningxia 750021;
2.Key Lab of Agricultural Biotechnology of Ningxia,Yinchuan,Ningxia 750002
Keywords:
Apocynum venetum L.; Trizol method; modified SDS method; RNA plant plus Reagent Kit method; extraction of total RNA
PACS:
S 563.7
DOI:
-
Abstract:
Total RNA was extracted by Trizol,modified SDS and RNA plant plus Reagent Kit methods,the extracted effect were compared by UV Spectrophotometry and Gel Electrophoresis,in order to screen out the best method for extracting Apocynum venetum L..The results showed that the RNA samples extracted by the method of Trizol were degraded gravely.Samples extracted by the method of modified SDS were low concentration and less amount,and the operation results were in poor repeatability.RNA plant plus Reagent Kit method was optimum extraction of A.venetum L. total RNA.The RNA samples extracted by the method of RNA plant plus Reagent Kit method were high quality,purity and good reproducibility,and could be used for research of molecule biology directly.

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Last Update: 2014-08-05