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Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年11期
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas
Author(s):
WANG Hong-lei; DING qiang; LV Wei; QU Wei; CUI Cong-guang; ZOU Ji-hua
Yantai Academy of China Agricultural University,Yantai,Shandong 264670
Keywords:
Agaricus bisporus; ISSR; genetic relationship; cluster analysis
PACS:
S 646
DOI:
-
Abstract:
The genetic relationship of 10 Agaricus bisporus cultivation strains was analyzed by inter-simple sequence repeats (ISSR).The results showed that 11 effective primers were screened from 117 ISSR primers.A total number of 73 clear bands were amplified from the 10 Agaricus bisporus strains,among which 51 bands were polymorphic and the polymorphic loci ratio was 69.86%.The genetic similarity coefficients among 10 strains were between 0.5479 and 0.9589 using NTSYSpc 2.10.The clustering analysis conducted with UPGMA indicated that 10 Agaricus bisporus strains could be divided into four groups:the first group included two domestic strains i.e.258 and 2796;the second group only included HRLM from Netherlands;the third group was consisted of L901,5113,XXX and 2200 from American;the fourth group was consisted of H901,F3F and HA15 from Netherlands.In contrast to two domestic strains,the genetic relationship of Agaricus bisporus strains between from American and from Netherlands was closer and the genetic similarity coefficients was higher than 0.85,but the genetic similarity of Agaricus bisporus from China was only 0.61.The introduction of foreign high-quality Agaricus bisporus strains would play an important role to increase Agaricus bisporus genetic diversity in our country.

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Last Update: 2014-08-05