Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年10期

Page:

80-84

Research Field:

Publishing date:

Info

Title:

Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim

Author(s):

ZHANG Zhen-xia¹; LI Cai-ping¹; ZHANG Qiu-mei¹; DONG Ting-xia²; TSIM Karl Wah-keung²; ZHENG Yu-zhong¹

1.Department of Biology，Hanshan Normal University，Chaozhou，Guangdong 521041；

2.Center for Chinese Medicine R & D，Hongkong University of Science and Technology，Hongkong 999077

Keywords:

Fritillaria przewalskii Maxim; tissue culture; bulb; proliferation

PACS:

S 567.23+.1

DOI:

-

Abstract:

In order to establish a rapid reproduction system of Fritillaria przewalskii Maxim，using bulb of Fritillaria przewalskii as material,the sterilization methods were optimized，combination of different plant hormones on the induced proliferation of bulbs from F.przewalskii were studied.The results showed that the procedure of optimal sterilization method were that，the materials should be immersed in 70% alcohol for 15 s，immerged in 0.1% mercuric chloride for 10 min，rinsed with sterile water 5 times，place in 4℃ for overnight；besides，the procedure should be repeated on the second day.Following above-mentioned steps，contamination rate of the materials was only 16.7%.The optimal culture medium that could induce bulbs of F.przewalskii to adventitious buds was MS+NAA 2.0 mg/L+6-BA 0.5 mg/L,and the induction rate had reached to 87.5%.The best subculture medium was MS+NAA 0.5 mg/L+6-BA 2.0 mg/L，and the multiplication coefficient was 9.The best rooting medium was 1/2MS+NAA 0.5 mg/L，and the rooting rate was about 90%.The paper had established regeneration system of F.przewalskii，which could provide the basic research reference for high-performance seedling methods of F.przewalskii.

References:

[l]国家药典委员会，中华人民共和国药典（一部）[M]．北京：中国医药科技出版社，2010．
[2]王书军，高文远，于琳，等．百合科贝母属药用植物分类研究进展[J]．中国中药杂志，2007，32(16)：1609-1614．
[3]李隆云，周裕书，代敏，等．暗紫贝母鳞茎再生组织培养技术研究[J]．中国中药杂志，1995，20(2)：78．
[4]张寿文，刘贤旺．贝母属植物的组织培养研究进展[J].江西农业大学学报，2003，25 (2)：187-190．
[5]马吉义．暗紫贝母的组织培养和植株再生[D].西宁：青海师范大学,2011．
[6]王鹤娉，安力，陈垣，等，岷贝母小鳞茎再生诱导效应的研究[J]．甘肃农业大学学报，2009，44(3)：50-52．
[7]高燕，贺宾，王艳，等，贝母愈伤组织的诱导及植株再生[J].新疆农业科学，2005，42(1)：35-37．
[8]李余先，陈凯锋，平贝母鳞茎离体培养研究[J].北方园艺，2010(3)：121-122．

Memo

Memo:

-

Common functions