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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年10期

Page:

84-87

Research Field:

Publishing date:

Info

Title:

Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng

Author(s):

WANG Yu-jiao¹; YANG Zhong²; ZHAO Chun-li¹; DONG Ran¹; ZHAO He-xiang¹; GU De-feng¹

1.College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118；2.Dehui Agricultural Science and Technology Education Center，Dehui，Jilin 130399

Keywords:

Pulsatill dahurica（Fisch.）Spreng; aseptic seedlings; plant growth hormone; tissue culture

PACS:

S 567

DOI:

-

Abstract:

Using aseptic shoots from seed of Pulsatill dahurica（Fisch.）Spreng as explants，tissue culture and rapid propagation of Pulsatill dahurica（Fisch.）Spreng were established and studied.The results showed that the seed of Pulsatill dahurica（Fisch.）Spreng optimum sterilization time was 4 min by 0.1% HgCl₂；the best combination of culture medium was MS+IAA 0.1 mg/L；optimum induced medium was MS+2,4-D 0.15 mg/L+6-BA 1.0 mg/L；optimal proliferation medium was MS+IAA 1.0 mg/L+ZT 0.2 mg/L；the rooting medium was 1/2MS+NAA 2.0 mg/L,the growth was the best;different medium (grass ashes,garden soil,and its equal ratio) had little influence on survival rate of transplanting,all above 90%.

References:

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[2]傅沛云，东北植物检索表[M]．2版，北京：科学出版社，1995.
[3]黄泰康，尤启冬，史美瑶，等．常用中药成分与药理手册[M].北京：中国医药科技出版社，1994:743．
[4]宋立人，胡烈，章国镇，等．中华本草[M]．7卷．上海：上海科学技术出版社，1999:239．
[5] Ye W C, Zhao S X, Cai H, et al. Studies on the chemical constituents o Pulsatilla chinensis [J].Chinese Chemical Letters,1991,2(5):375.
[6]钟长斌，李祥，白头翁的化学成分及药理作用研究述要[J].中医药学刊，2003，21(8)：138-139．

Memo

Memo:

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Last Update: 2014-08-06