Optimization of Culture Conditions on the Liparis Mycorrhizal Fungi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Culture Conditions on the Liparis Mycorrhizal Fungi

Author(s):: ZHANG Yi-zihan¹; 2; ZHANG Qun²; WANG Ping-ping²; LI Hai-yan³; ZHANG Li-jie²; QU Bo²; 1.College of Life Science,Inner Mongolia University,Hohhot,Inner Mongolia 010018;
2.College of Biological Science and Technology,Shenyang Agricultural University,Shenyang,Liaoning 100866；
3.Liaoning Fushun Laotudingzi National Nature Reserve Administrations,Xinbin,Liaoning 113208

Abstract:: Taking Liparis arethusa mycorrhizal fungi as material,through separation,purification and cultured,using orthogonal experimental method to set culture conditions that include temperature,carbon source,nitrogen source and medium pH value,the Liparis ?mycorrhizal fungi culture system which based on the single-factor test were established.The results showed that the white or pale yellow colonies of Liparis ?mycorrhizal fungi showed the uniform radial outward growth,growth surface of sloppy or rich aerial hyphae with transparent droplets.Through measurement and analysis of colony growth rate,four factors best level were set the carbon source was concentration of 2% glucose,the nitrogen source was concentration of 0.5% ammonium chloride,pH value of 7,temperature of 25℃.Colony growth rate decreased at faster speed,with the increase in the number of days,and finally tended to a constant value in the tenth.

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Last Update: 2014-08-13