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Molecular Cloning and Expression Analysis of Catalase (PuCAT) Gene in Puccinellia chinampoensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年20期
Page:
90-95
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Expression Analysis of Catalase (PuCAT) Gene in Puccinellia chinampoensis
Author(s):
REN Wei 1; 2; XU Bo3; GENG Hui1; WANG Zhi-feng1; XU An-kai1
1.Animal Science Branch,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100；
2.Institute of Soil and Water Conservation,Chinese Academy of Sciences,Yangling,Shaanxi 712100；
3.Jilin Agricultural University,Changchun,Jilin 130000
Keywords:
Puccinellia chinampoensis; CAT; gene clone
PACS:
Q 785
DOI:
-
Abstract:
The full length cDNA sequence of CAT gene was cloned from Puccinellia chinampoensis leaves using RT-PCR method,the primers were designed according to the homologous CAT gene sequences of other plant species.The results showed that the nucleotide sequence of the gene was 1 487 bp,containing a complete open reading frame and encoding 492 amino acids,Genebank：HM230827.Nucleotide and amino acid sequence analysis revealed that PuCAT shared high identity with the orthologs from Triticum aestivum and Hordeum vulgare.And its signal peptide,hydrophobicity/hydrophilic,trans-membrane domain,secondary structure and main functional domains were predicted.Semi-quantitative RT-PCR analysis showed that PuCAT expressed in different tissues,but the expression in root was lower,and in leaf much higher.Various elevated levels of PuCAT expression had been detected when exposed to 4 different stress experimental treatments,and the results were not the same.

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Last Update: 2014-11-21