|Table of Contents|

Establishment of the Efficient Regeneration System of Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年20期
Page:
96-100
Research Field:
Publishing date:

Info

Title:
Establishment of the Efficient Regeneration System of Cucumber
Author(s):
LI Wen-lu; JIANG Shou-yang; ZHANG Nan; CHENG Xiao-jing; LI Xiang; YANG Zheng-an; ZHANG Ying-hua
College of Landscape and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201
Keywords:
cucumber; cotyledon node; adventitious buds; regeneration system
PACS:
S 642.2
DOI:
-
Abstract:
Taking cotyledons,cotyledon node and hypocotyl of 20 kinds of cucumber cultivated cultivars as material,the germination rate,callus and induced differentiation of adventitious buds were tested,the most suitable for cucumber varieties of regeneration system was established.The results showed that the highest germination rate were 66.7% and 83.3% for ‘Nongjiale’and ‘Shennongchunsi F1’ cucumber;the cotyledon node germination rate was the best in the medium of MS+0.5 mg/mL 6-BA for different type explants；‘Nongjiale’ and ‘Shennongchunsi F1’ cucumber in the medium of MS+0.5 mg/L 6-BA+1.0 mg/L AgNO3 got the highest bud value-added factor and the induction rate were 50.0% and 56.3%.According to the germination rate,callus induction and bud differentiation in different varieties,the fast and high frequency regeneration system in cucumber was established.The study would provide the technological basis for the gene engineering of cucumber.

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Last Update: 2014-11-21