|Table of Contents|

Biological Characteristics of the Leaf Spot Lesions of Osmanthus fragrans and Its Resticide Sensitivity(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年19期
Page:
117-120
Research Field:
Publishing date:

Info

Title:
Biological Characteristics of the Leaf Spot Lesions of Osmanthus fragrans and Its Resticide Sensitivity
Author(s):
LI Yong-li; ZHOU Zhou; YUAN Chun-yan; DUAN Pei-lin
College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003
Keywords:
Botryosphaeria dothidea; sweet osmanthus leaf spot; biological characteristics; toxicity
PACS:
S 432.1
DOI:
-
Abstract:
Taking sweet osmanthus as material,tissue isolation method was conducted to isolate the pathogen.The pathogen was identified by the morphological characters and its internal transcribed spacer (ITS) sequence of rDNA.Meantime the biological characteristics of the pathogen and its sensitivity to nine fungicides were studied.The results showed that the pathogen was Botryosphaeria dothidea.The influence of illumination on the growth of hyphae was not significant.The optimal temperature for the growth of hyphae was 20~30℃ and the optimal pH value was 6~9.The best carbon sources were glucose,sucrose and maltose.The best nitrogen sources were KNO3 and asparagine.All of nine fungicides could inhibit the growth of hyphae,the inhibitory activities of difenoconazole,tebuconazole and flusilazole were the highest with EC50 of 0.6839,0.8754,1.2236 μg/mL,and the inhibitory activities of chlorothalonil was the lowest with EC50 of 57.0778 μg/mL.

References:

[1]郭桢,陈永辉,姜子德.广州地区观赏植物真菌病害鉴定初报[J].广东农业科学,2004(4):45-47.
[2]袁蒲英,朱天辉.桂花叶枯病病原菌鉴定及生物学特性研究[J].四川林业科技,2008,29(4):21-28.
[3]谢玲,唐晨光,岑贞陆,等.广西桂花叶枯病病原菌鉴定及其生物学特性研究[J].西南农业学报,2009,22(5):1358-1362.
[4]罗萍,朱天辉.桂花叶枯病调查及病原的鉴定[J].四川林业科技,2008,29(5):30-33.
[5]朱天辉.园林植物病理学[M].北京:中国农业出版社,2003:168-169.
[6]王晓梅,黄琦,李玉.桂花叶斑病病原鉴定及其生物学特性研究[J].吉林农业大学学报,2011,33(2):151-157.
[7]Xie L,Huang S L,Cen Z L,et al.First report of Botryosphaeria dothidea causing sweet osmanthus leaf dieback in China[J].Agricultural Sciences in China,2010,9(6):847-853.
[8]方中达.植病研究方法(2版)[M].北京:中国农业出版社,1979:189-190.
[9]杨焕青,王开运,范昆,等.草莓枯萎病菌的生物学特性及7种杀菌剂对其抑制作用[J].植物保护学报,2008,35(2):169-174.
[10]宋微,孟繁荣.落叶松根朽病生理学特性及其拮抗菌的测定[J].南京林业大学学报（自然科学版）,2007,31(1):67-71.
[11]郑洪波,郑翠梅,贾玉.烟草弯孢炭疽病病原鉴定及生物学特性[J].植物保护学报,2012,39(2):189-190.
[12]Van N J M,Crous P W,Groenewald J Z,et al.DNA phylogeny,morphology and pathogenicity of Botryosphaeria species on grapevines.Mycologia,2004,96(4):781-798.
[13]Urbez T J R,Leavitt G M,Voegel T M,et al.Identification and distribution of Botryosphaeria spp.associated with grapevine cankers in California[J].Plant Disease,2006,90(12):1490-1503.
[14]Pitt W M,Huang R,Steel C C,et al.Identification,distribution and current taxonomy of Botryosphaeriaceae species associated with grapevine decline in New South Wales and South Australia[J].Australian Journal of Grape and Wine Research,2010,16(1):256-271.
[15]赵娜,张伟伟,高小宁,等.中国部分省份苹果轮纹病菌及相关类群的系统学关系[J].西北农林科技大学学报（自然科学版）,2011,39(10):123-133.
[16]张会领,段银昌,胡秀丽,等.杨树溃疡病的发生与防治[J].植物保护,2012(8):20-21.
[17]罗卿权,徐颖,刘美.红瑞木溃疡病病原菌的鉴定[J].植物保护,2012,38(4):115-117.
[18]张勇,李晓军,曲健禄,等.扁桃流胶病病原菌及其生物学特性研究[J].中国果树,2008(1):41-44.

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Last Update: 2014-11-25