|Table of Contents|

Influence Factors of Meristem Culture,Shoot Induction and Multiplication of Pugionium cornutum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年19期
Page:
107-112
Research Field:
Publishing date:

Info

Title:
Influence Factors of Meristem Culture,Shoot Induction and Multiplication of Pugionium cornutum
Author(s):
KANG Li-ru; SHI Ling; ZHANG Qi-li; TIAN Rong-wei; ZHANG Dong-hui; HAO Meng-jie
College of Agronomy,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019
Keywords:
Pugionium cornutum; apical meristem; axillary; induction; proliferation
PACS:
S 54
DOI:
-
Abstract:
Taking aseptic seed-derived plantlets as explants,effect of light intensity on meristem culture,effect of concentration of 6-BA and IBA on shoot inducement and multiplication of P.cornutum and effect of disinfection method on filed explants were also studied.The results showed that the optimum light intensity for culturing apical meristem of P.cornutum was 3 600 lx;the optimum medium of stem segments explants the basal B5 medium supplemented with 5.0 mg/L 6-BA and 0.5～1.0 mg/L IBA,the generation rate was 93.3%～96.7% and the average number of buds induction was 4.2～4.3；the highest shoot proliferation rate of 3.44 times was obtained on the medium of B5+6-BA 5.0 mg/L+IBA 1.0 mg/L,shoot clusters induction rate could reach 93.1%;a 30 seconds disinfection time with a 70% alcohol solution,and then 0.1% HgCl2 for 10～12 minutes were the best disinfection method.The contamination rate was 10%,the survival frequency reached to 85%,browning rate was only 20%.However for longer growth periods and lower shoot proliferation capacity,these explants were not suitable for the propagation of P.cornutum.

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Last Update: 2014-11-25