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Assessment of Genetic Diversity Among Fusarium Wilt Pathogens of the Cucurbits Detected by ISSR Markers(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年17期
Page:
97-101
Research Field:
Publishing date:

Info

Title:
Assessment of Genetic Diversity Among Fusarium Wilt Pathogens of the Cucurbits Detected by ISSR Markers
Author(s):
SU Jia; GAO Zeng-gui; YAO Yuan; LIU Xian; WANG Xiao-dong; LI Wan-ying
Institute of Plant Immunology,Shenyang Agricultural University,Shenyang,Liaoning 110866
Keywords:
Fusarium oxysporum; ISSR-PCR; genetic diversity
PACS:
S436.42
DOI:
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Abstract:
Genetic variation among 32 Fusarium oxysporum isolates was analysed by using inter-simple sequence repeat (ISSR) polymorphism,forma specialis of Fusarium oxysporum Schl.were researched from the perspective of genetic diversity.The results showed that a total of 109 bands were amplified with 11 ISSR primers,99 bands were polymorphic,the rate was 90.82%.The amplified fragment sizes ranged from 120 to 2 000 bp.The genetic similarity among 32 F.oxysporum isolates ranged from 0.64 to 0.91 and all the strains could be divided into 5 groups at the level of 0.705.In the same group,there was no distinct relation between the genetic similarity and geographic origin of the strains isolated from the same hosts.

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Last Update: 2014-09-26