Study on the Isolation of Endophytic Fungus from Solanum nigrum L. and Its Microbial Inhibition Activity(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年21期

Page:

161-165

Research Field:

Publishing date:

Info

Title:

Study on the Isolation of Endophytic Fungus from Solanum nigrum L. and Its Microbial Inhibition Activity

Author(s):

BI Jiang-tao¹; YANG Zhi-wei²; HUANG Pan-pan³; WANG Jing¹; GUAN Xiao-qing²

1.Research and Development Center for New Techniques，Ningxia University，Yinchuan，Ningxia 750021;

2.School of Agriculture，Ningxia University，Yinchuan，Ningxia 750021;

3.College of Life Sciences，Ningxia University，Yinchuan，Ningxia 750021

Keywords:

medicinal plant; Solanum nigrum; endophytic fungi; isolation; microbial inhibition activity

PACS:

Q 93;S 567

DOI:

-

Abstract:

Taking medicinal plant Solanum nigrum L.as material，the endophytic fungi strains were isolated from root，stem and leaf of the host by tissue inoculation culture and five pathogenic fungi and four bacteria strains were used as indicators to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods in order to explore the resource diversity and microbial inhibition activity of endophytic fungi from it.The results indicated that twenty-nine fungal endophytic strains were isolated from the host，most of them coming from root，then from leaf and stem；the isolated strains attributed to four genera，two families，and two orders based on morphological characteristics；79.3% of the total isolated strains were found to have some microbial inhibition activities against two or more indicating fungi，41.4% of the total isolates had antibacterial activities against one or more indicating bacteria；the strain ‘SNEFL012’ had evident inhibition activities to four kinds of pathogenic indicators，and the strain ‘SNEFS005’ had evident inhibition activities to two kinds of bacterial indicators and two kinds of pathogenic indicators，the two bioactive strains with evident antimicrobial activities belonging to Aspergillus sp.and Fusidium sp.，respectively.The medicinal plant Solanum nigrum L.showed relatively abundant endophytic fungal resource for pathogenic inhibition，and its microbial inhibition was worthwhile for further studies.

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