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Analysis of Phytochelatin Synthase Genes’ Characteristics in Different Plants(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年21期
Page:
110-116
Research Field:
Publishing date:

Info

Title:
Analysis of Phytochelatin Synthase Genes’ Characteristics in Different Plants
Author(s):
JIANG Qian-qian; CAO Hui
Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong，Weifang University，Weifang，Shandong 261061
Keywords:
phytochelatin synthase(PCS); physical and chemical properties; phylogeny; sequence and structure analysis
PACS:
Q 946.5
DOI:
-
Abstract:
Phytochelatin synthase (PCS) is the key enzyme which catalyzes glutathione (GSH) polymerization to generate phytochelatins (PCs) in plants，and plays an important role in the mitigation of heavy metal stress.The nucleic acid and amino acid sequences，physical and chemical properties，protein structure，phylogenetic trees and functional domains of PCS genes from Malus domestica,Malus hupehensis，as well as Pyrus betulifolia，Arabidopsis thaliana，Oryza sativa，Nicotiana tabacum，and Lotus japonicas which had been registered in GenBank were analyzed and investigated using bioinformatics online methods and software，such as ProtParam，TMHMM，SignalP，Phyre2，Pfam，Clustal X and MEGA.The results showed that the length of PCS proteins was between 465~506 amino acids，and the isoelectric point was between 5.67~7.77.The PCS proteins were mainly localized in the nucleus.The plant PCS proteins were unstable protein except Ceratophyllum demersum.The secondary structure of PCS proteins were mainly composed of α-helix，extended strand and random coil，and their spatial structure had a high degree of similarity.All the PCS proteins belonged to phytochelatin synthases protein families，which containing a phytochelatin functional domain，and three predicted active sites.This study would provide the basis for further investigating the structural characteristics and functions of this enzyme.

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