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Isolation and Expression Analysis of Sucrose Synthase Gene cDNA from Lycium barbarum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年21期
Page:
105-109
Research Field:
Publishing date:

Info

Title:
Isolation and Expression Analysis of Sucrose Synthase Gene cDNA from Lycium barbarum L.
Author(s):
WANG Li-juanSHI JingWANG Yan-cai
College of Life Science,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
Lycium barbarum L.sucrose synthasegene cloningexpression analysis
PACS:
S 567.1+9
DOI:
-
Abstract:
Taking ‘Ningqi No.1’ as material,the primers were designed according to the conserved domains of the plant sucrose synthase genes’ nucleotide sequences,and a sucrose synthase gene was cloned from Lycium barbarum L.by RT-PCR method.The full length of Susy gene was cloned by RACE.Its biological information was analyzed and gene expression levels in different organs were tested using Real-time PCR.The results showed that the fragment cloned named Lb Susy (GenBank number:KC907702) was 2 926 bp in length.The amino acid sequences were 84%~100% identical to the sequences of tomato,Alnus glutinosa ?etc.The putative protein encoded by Lb Susy was gone to mitochondrion by PSORT analysis software.Real time PCR analysis showed that the Lb Susy gene was highly expressed in stem.The expression level reached the lowest level in root.

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Last Update: 2014-08-26