|Table of Contents|

Cloning and Sequence Analysis of Aquaporins Gene in Chimonanthus praecox cv.Luteus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年21期
Page:
99-104
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of Aquaporins Gene in Chimonanthus praecox cv.Luteus
Author(s):
FANG Zi-yi; SONG Xiao-xi; ZHAO Shi-ping; WANG Qian-li; ZHAO Kai-ge; CHEN Long-qing
Key Laboratory of Horticultural Plant Biology of Ministry of Education，College of Horticulture and Forestry Science，Huazhong Agricultural University，Wuhan，Hubei 430070
Keywords:
Chimonanthus praecox cv.Luteus; aquaporins; gene cloning; sequence analysis
PACS:
S 685.99
DOI:
-
Abstract:
Taking Chimonanthus praecox cv.Luteus as material，a pair of primers were designed according to the gene sequence in the transcriptome database created，the gene encoding aquaporins in sepals of Chimonanthus praecox at peak flowering stage was cloned by PCR and its molecular structure of amino acid was analyzed.Based on sequence alignment in cDNA database and using bioinformatics，the coding sequence，protein structure and evolutionary relationships were analyzed.The results showed that a 2 264 bp gene encoding aquaporins was amplified successfully from genomic DNA of petals of Chimonanthus praecox at peak flowering stage，being named CpAQP3.The gene CpAQP3 consisted a 5′untranslated region (UTR) of 50 bp，a single long open reading frame (ORF) of 2 049 bp，and a 3′UTR of 165 bp.The ORF encoded 284 amino acids.This gene include two introns of 708 bp and 594 bp and three exons of 127 bp，250 bp and 370 bp respectively.An aquaporin gene CpAQP3 was firstly cloned from Chimonanthus praecox.The CaAQP gene was a member of the TIP subgroup in MIP protein family and had the typical function region.The CpAQP3 gene may play an important regulating water transportation role.There were significant differences in sequential structure between this gene and the other known aquaporin gene from Xinkou Chimonanthus praecox.

References:

[1]Maurel C，Verdoucq L，Luu D T，et al.Plant aquaporins：membrane channels with multiple integrated functions[J].Annu Rev Plant Biol,2008,59:595-624.

[2]Johansson I，Karlsson M，Johanson U，et al.The role of aquaporins in cellular and whole plant water balance[J].Biochimica Biophysica Acta,2000,1465(1-2):324-342.

[3]Denker B M，Smith B L，Kuhajda F P，et al.Identification,purification，and partial characterization of a novel Mr 28,000integral membrane protein from erythrocytes and renal tubules[J].J Biol Chem,1988,263(30):15634-15642.

[4]Preston G M，Carroll T P，Guggino W B，et al.Appearance of waterchannels in Xenopus oocytes expressing red cell CHIP28 protein[J].Science,1992,256(5055):385-387.

[5]Maurel C，Reizer J，Schroeder J I，et al.The vacuolar membraneprotein γ-TIP creates water specific channels in Xenopus oocytes[J].Embo J,1993,12(6):2241-2247.

[6]Ludewig U，Dynowski M.Plant aquaporin selectivity：wheretransport assays，computer simulations and physiology meet[J].Cell Mol Life Sci,2009,66(19):3161-3175.

[7]Secchi F，Lovisolo C，Uehlein N，et al.Isolation and functional characterization of three aquaporins from olive (Olea europaea?L.)[J].Planta,2007,225:381-392.

[8]Sakurai J，Ishikawa F，Yamaguchi T，et al.Identification of 33 rice aquaporin genes and analysis of their expression and function[J].Plant Cell Physiology,2005,46:1568-1577.

[9]Maurel C，Verdoucq L，Luu D T，et al.Plant aquaporins：membrane channels with multiple integrated functions[J].Annu Rev Plant Biol,2008,59:595-624.

[10]Mahantesha N，Khader S，Oommen K，et al.STIFDB2：an updated version of plant stress-responsive transcription factor database with additional stress signals，stress-responsive transcription factor binding sites and stress-responsive genes in arabidopsis and rice[J].Plant Cell Physiol,2013,54(2):1-15.

[11]Petersen T N，Brunak S，von Heijne G, et al.SignalP 4.0：discriminating signal peptides from transmembrane regions [J].Nature Methods,2011,8(10):785-786.

[12]于秋菊,吴铸,林忠平.植物水孔蛋白研究进展[J].北京大学学报(自然科学版),2002,38(6):855-866.

[13]阮想梅,李登弟,李学宝.植物水孔蛋白的功能和调控[J].植物生理学通讯,2009,45(1):1-7.

[14]Maurel C，Verdoucq L，Luu D T，et al.Plant aquaporins：membranechannels with multiple integrated functions[J].Annu Rev Plant Biol,2008,59:595-624.

[15]Fraysse L C，Wells B，McCann M C，et al.Specific plasma membrane aquaporins of the PIP1 subfamily are expressed in sieve elements and guard cells[J].Bio Cell,2005,97(7)：519-534.

[16]Sarda X，Tousch D，Ferrare K，et al.Two TIP-like genes encoding aquaporins are expressed in sunflower guard cells[J].Plant J,1997,12(5):1103-1111.

[17]张迷.蜡梅水通道蛋白基因CpAQP1和CpAQP2克隆及功能初步分析[D].重庆:西南大学,2010.

[18]Mourier T，Jeffares D C.Eukaryotic intron loss[J].Science,2003,300:1393.

[19]庞永奇,王高奇,王旭初,等.植物水孔蛋白的亚细胞分布与生理功能研究浅析[J].生物化学与生物物理进展,2012,39(10):962-971.

[20]Smart L B，Moskal W A，Cameron K D，et al.MIP genes are down-regulated under drought stress in Nicotiana glauca[J].Plant and Cell Physiology,2001,42(7):686-693.

[21]陈儒钢,朱文超,巩振辉,等.辣椒水通道蛋白基因CpAQP3 的克隆与序列分析[J].中国农业科学,2010,43(20):4323-4329.

[22]刘祥.蜡梅水通道蛋白CpAQPl转录水平变化及其启动子活性分析[D].重庆:西南大学,2012.

[23]Paul E V，Manu A，Surekha K A，et al.Methods and concepts in quantifying resistance to drought，salt and freezing，abiotic stresses that affect plant water status[J].The Plant Journal,2006,45:523-539.

[24]Aroca R，Amodeo G，Fernandez-Illescas S，et al.The role of aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots[J].Plant Physiol,2005,137:341-353.

[25]Guenther J F，Nouth C，Galetovic M P，et al.Phosphorylation of soybean nodulin 26 on serine 262 enhances water permeability and is regulated developmentally and by osmotic signals[J].The Plant Cell,2003,15:981-991.

[26]Azad A K，Sawa Y，Ishikawa T，et al.Phosphorylation of plasma membrane aquaporin regulates temperature-dependent opening of tulip petals[J].Plant Cell Physiol,2004,45:608-617.

[27]Azad A K，Katsuhara M，Sawa Y，et al.Characterization of four plasma membrane aquaporins in tulip petals：a putative homolog is regulated by phosphorylation[J].Plant Cell Physiol,2008,49:1196-1208.

[28]Maeshima M，Ishikawa F.ER membrane aquaporins in plants[J].Pflugers Archive-European Journal of Physiology,2008,456(4):709-716.


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Last Update: 2014-08-26