Study on in vitro Culture of Forced Shoots of Quercus mongolica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年19期

Page:

102-105

Research Field:

Publishing date:

Info

Title:

Study on in vitro Culture of Forced Shoots of Quercus mongolica

Author(s):

GAO Shan¹; LIN Mei¹; CUI Jian-guo¹; LIN Qiang²; LIU Jing-yu³

1.College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866；

2.Forestry Technology Extension and Training Center of Benxi City，Benxi，Liaoning 117022；

3.Forestry Science Institute of Benxi City，Benxi，Liaoning 117022

Keywords:

Quercus mongolica; forced shoot; tissue culture; plant regeneration

PACS:

S 792.186

DOI:

-

Abstract:

Taking the forced shoots of Quercus mongolica as a source of initial explants，the effects of different types of media and different concentrations of plant growth regulators and their ratios on organogenesis and plantlet regeneration of Quercus mongolica were studied.The results showed that regenerated plants could be obtained from forced shoots of Quercus mongolica via organogenesis.The best initial medium was WPM+6-BA 0.5 mg/L+2,4-D 0.5 mg/L,the induction rate was 76.7%.The best adventitious bud proliferation medium was WPM+6-BA 2.0 mg/L+NAA 0.5 mg/L and the highest proliferation factor was 3.5.The best rooting medium was 1/2MS+NAA 0.5 mg/L+IBA 0.1 mg/L and the rooting rate was up to 76.7%.

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