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Study on Agrobacterium mediated Inter leukin4 Gene Transferred into Cabbage(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年15期
Page:
113-116
Research Field:
Publishing date:

Info

Title:
Study on Agrobacterium mediated Inter leukin4 Gene Transferred into Cabbage
Author(s):
JIN Xiao-xia; YU Hai-feng; YU Li-jie
College of Life Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025
Keywords:
 ; Brassica oleracea L.; IL-4; genetic transformation
PACS:
S 635
DOI:
-
Abstract:
Taking the cabbage inbred lines seeds of transgenic IL-4 T0 materials named ‘92-1012-3-11’ as experimental material，the experiment researched the genetic transformation of IL-4gene into cabbage,which was mediated by Agrobacterium tumefaciens.The results showed that 396 strains of PPT resistant regenerated plants,137 strains of PCR positive plants,wherein the cotyledons with petiole conversion rate of 8.1%,hypocotyls differentiation rate was 5.8%.IL-4 positive cabbage plant in the positive transgenic line T0generation were detected by PPT resistance,PCR,PCRSouthern hybridization,and was showed that the IL-4 gene had been transferred into cabbage receptor.IL-4 gene had been integrated into the genome of cabbage which were detected by ELISA and Western detection,but it expressed very low (0.23~2.81 ng/mL),and there were difference greatly between the expression of each plant.

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Last Update: 2014-08-12