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Study on Cloning and Expression of atp9 Gene in Carrot and Its Relationship to Cytoplasmic Male Sterility(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年24期
Page:
140-144
Research Field:
Publishing date:

Info

Title:
Study on Cloning and Expression of atp9 Gene in Carrot and Its Relationship to Cytoplasmic Male Sterility
Author(s):
ZHANG Zhi12LIANG Yi1DING Hai-feng1ZHANG Hong-wei1
1.Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China,Beijing Jingyan Yinong Sci-Tech Developent Center,Beijing 100097;
2.Shanxi Agriculture University,Taigu,Shanxi 030800
Keywords:
carrotatp9 genecytoplasmic male sterilityRT-PCRreal-time PCR
PACS:
S 631.2
DOI:
-
Abstract:
Specific primers were designed according to the atp9 gene in carrot and other plants,carrot mitochondrion gene fragments associated with cytoplasmic male sterility were cloned either.The results showed that carrot atp9 gene contains a many base pair’s substitution and deletion in sterile line compared with maintainer line.The expression analysis using semi-quantitative RT-PCR and Real-time PCR demonstrates that expression of atp9 gene in carrot sterile line was significantly lower than in maintainer line besides the big bud stage.These researches indicated that atp9 gene mutation and its abnormal expression had a closely relationship with carrot cytoplasmic male sterility.

References:

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Last Update: 2014-09-01