Advance and Prospects on Salt-tolerant Transgenge Alfalfa(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年19期

Page:

184-188

Research Field:

Publishing date:

Info

Title:

Advance and Prospects on Salt-tolerant Transgenge Alfalfa

Author(s):

LI Hui-wen¹; MA Dong-mei²; XU Xing¹; 2

1.College of Life Science，Ningxia University，Yinchuan，Ningxia 750021;

2.College of Agriculture，Ningxia University，Yinchuan,Ningxia 750021

Keywords:

alfalfa; salt-tolerant; transgene; advance

PACS:

S 551.+7

DOI:

-

Abstract:

Salinization of the soil greatly affects the growth of alfalfa.In this paper，the research progress through genetic engineering to improve the ability of alfalfa salinity and some new salt-tolerant alfalfa material obtained through genetic engineering methods were reviewed,and some problems in currently study of salinity tolerance of transgenic alfalfa were analyzed,the future research trend was prospected.

References:

[1]Sengupta-Gopalan C，Bagga S，Potenza C，et al.Alfalfa[J].Biotechnology in Agriculture and Forestry,2007，61:321-335.

[2]梁云媚,多立安,李燕,等.不同盐分胁迫对苜蓿种子萌发的影响[J].草业科学,1998,15(6):21-25．

[3]Saunders J W,Bingham E T.Production of alfalfa plants from callus tissue[J].Crop Sei,1972,12:804-808.

[4]Deak M,Kissv.Agrobacterium mediated gene transfer[J].Plant Cell ReP,1986(5):97-100.

[5]Winieov I,Bastola C R.Transgenic overexpression of the transeription Faetor Alfinl enhances expression of the endogenous MsPRP2 gene in alfalfa and improves salinity tolerance of Plants[J].Plant Phys,1990,120:473-480.

[6]陈晨,乔代蓉,白林含,等.农杆菌介导的杜氏盐藻Dscbr基因转化紫花苜蓿的初步研究[J].四川大学学报(自然科学版),2005,42(3):567-570.

[7]梁慧敏,夏阳,孙仲序,等.根癌农杆菌介导苜蓿遗传转化体系的建立[J].农业生物技术学报,2005,13(2):152-156.

[8]燕丽萍,夏阳,毛秀红,等.转BADH基因紫花苜蓿山苜2号品种的抗盐性鉴定及系统选育[J].植物学报,2011(3):63-71.

[9]马晖玲.紫花苜蓿抗旱耐盐碱转基因植株选育的研究[D].兰州:甘肃农业大学,2004.

[10]晏石娟,马晖玲,曹致中.紫花苜蓿抗早耐盐碱转基因抗性苗耐盐性研究[J].甘肃农业大学学报,2006,10(5):91-94.

[11]王强龙,王锁民,张金林,等.根癌农杆菌介导AtNHX1基因转化紫花苜蓿的研究[J].草业科学,2006,23(12):55-59.

[12]邓程君.农杆菌介导的NTHK1基因转化苜蓿增大叶片及提高耐盐性的研究[D].北京:中国农业大学,2006.

[13]隋昕.碱蓬液泡型Na+/H+逆向运输蛋白基因的克隆及其向苜蓿中的转化[D].长春:吉林农业大学,2007.

[14]潘竟丽,张富春.农杆菌介导灰绿黎Cg-NXH1基因转化新疆大叶苜蓿的研究[J].新疆农业科学,2007,44(4):418-422.

[15]赵红娟,张博,李培英.农杆菌介导犁苞滨藜NHX基因转化苜蓿的影响因素[J].草地学报,2007,15(5):418-422.

[16]盛慧,朱延明,李杰,等.DREB2A基因对苜蓿遗传转化的研究[J].草业科学,2007,24(3):40-45.

[17]李世林,杨舸,易秀莉,等.根癌农杆菌介导的盐生杜氏藻DsNRT2基因转化紫花苜蓿的初步研究[J].四川大学学报(自然科学版),2008,45(2):409-412.

[18]刘小琳,康俊梅,孙彦,等.MsNHX1基因转化紫花苜蓿及转基因植株的鉴定[J].草地学报,2008(2):115-120.

[19]王玉祥,王涛,张博.转rstB基因苜蓿耐盐性初评(简报)[J].草地学报,2008,16(5):539-541.

[20]Bao A K，Wang S M，Wu G Q,et al.Overexpression of the Arabidopsis H+-PPase?enhanced resistance to salt and drought stress intransgenic alfalfa(Medicago sativa?L.) [J].Plant Science,2009,176:232-238.

[21]Ramón Suárez，Cecilia Calderón，Gabriel Iturriaga.Enhanced Tolerance to Multiple Abiotic Stresses in Transgenic Alfalfa Accumulating Trehalose[J].Crop Science，2009,49:1791-1799.

[22]Jin T C，Chang Q，Li W F，et al.Stress-inducible expression of GmDREB1 conferred salt tolerance in transgenic alfalfa[J].Plant Cell Tiss Organ Culture，2010:100:219-227.

[23]Liu Z H，Zhang H M，Li G L，et al.Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase[J].Euphytica,2011,178:363-372.

[24]Li W F，Wang D，Jin T C,et al.The Vacuolar Na+/H+ Antiporter Gene SsNHX1 from the Halophyte Salsola soda Confers Salt Tolerance in Transgenic Alfalfa (Medicago sativa L.) [J].Plant Mol Biol Rep,2011,29:278-290.

[25]Hua Y，Zhang B X，Cai H，et al.Stress-inducible expression of GsSAMS2 enhances salt tolerance in transgenic Medicago sativa[J].African Journal of Biotechnology，2012,11(17):4030-4038.

[26]Pereira L F,Eriekson L.Stable transformation of alfalfa by Partiele bombardment[J].Plant Cell RePorts,1995(2):290-295.

[27]Ramaiah S M,Skirmer D Z,Shashi M Ramaiah,et al.Particle bombardment：a simple and efficient method of alfalfa(Medicago sativa L.) Pollen transformation[J].Current Science,1997,73(8):674-682.

[28]王瑛,朱宝成,孙毅.外源lea3基因转化紫花苜蓿的研究[J].核农学报,2007,21(3):249-252.

[29]肖荷霞.苜蓿转化再生体系的建立及LEA3基因研究[D].石家庄:河北大学,2005.

[30]牛一丁,霍朝霞,哈斯阿古拉,等.紫花苜蓿花粉管通道法转基因技术初探[J].中国草地学报,2009,31(1):36-39.

[31]张立全,牛一丁,郝金凤,等.通过花粉管通道法导入红树总DNA获得耐盐紫花苜蓿T0代植株及其RAPD验证[J].草业学报,2011(3)：292-297.

[32]刘明,杨君,安利佳.花粉管通道法转化影响因素的分析[J].安徽农业科学,2002,35(12):3483-3523.

[33]许仕珍.紫花苜蓿不同再生体系的比较及农杆菌介导的AtNHX1基因转化研究[D].兰州:甘肃农业大学,2006.

[34]Reich T J,Lyer V N,Mikib L.Efficient Transformation of Alfalfa Protoplasts by the Intranuclear Microinjection of Ti-plasmids[J].Bio Technology,1986(4):1001-1004.

[35]Kuchuke N.Plant Genic Transfer [J].Plant Cell Rep,1990(8):660-663.

[36]张建军,金湘,徐建辉,等.氩离子注入介导甘草总DNA在阿尔冈金紫花苜蓿中转化的初步研究[J].种子,2008,27(4):57-59.

[37]杨起简,周禾,孙彦,等.紫花苜蓿的愈伤组织诱导及组织培养[J].北京农学院学报,2004,19(2):29-31.

[38]Samac D A.Strain specificity in transformation of alfalfa by Agrobacterium tumefaciens[J].?Plant Cell Tiss Org Cult,1995,43:271-277.

[39]黎茵,黄霞,黄学林.根癌农杆菌介导的苜蓿体胚转化[J].植物生理与分子生物学学报,2003,29(2):109-113.

[40]刘文婷,段琦梅,刘景玲,等.农杆菌介导的苜蓿次级体细胞胚的遗传转化[J].生物工程学报，2012,28(2):203-213.

[41]王超.农杆菌介导的碱蓬液泡膜H⁺-ATPase基因转化苜蓿的研究[D].长春:吉林农业大学,2011.

[42]Anthony T T,Stephen H B,Kardailsky V,et al.Transformation of Medicago truncatula via infiltration of seeding or flowering plants with Agrobacterium [J].Plant J,2000,22:531-541.

[43]马忠强,尹航.转基因技术在作物育种中的应用[J].现代化农业,2009(10):25-26.

[44]Ferradini N，Nicolia A，Capomaccio S,et al.Assessment of simple marker-free genetic transformation techniques in alfalfa[J].Plant Cell Rep,2011,30(11):1991-2000.

[45]王相春,程在全,曾千春,等.植物筛选标记基因应用进展[J].安徽农业科学,2011,39(22):13290-13291,13365.

[46]Malik K,Wu K,Li X Q,et al.A constitutive gene expression system derived from the tCUP cryptic promoter elements[J].Theor Appl Genet,2002,105:505-514.

[47]Khoudi H，Vézina L P，Mercier J，et al.An alfalfa rubisco small subunit homologue shares cis-acting elements with the regulatory sequences of the RbcS-3A gene from pea[J].Gene,1997,197:343-351.

[48]Kasuga M，Liu Q，Miura S，et al.Improving plant drought，salt，and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nat Biotechnol,1999,17:287-291.

Memo

Memo:

-

Common functions