Cloning and Aequence Analysis of ANS Gene from Litchi chinensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年19期

Page:

118-121

Research Field:

Publishing date:

Info

Title:

Cloning and Aequence Analysis of ANS Gene from Litchi chinensis

Author(s):

ZHAO Zhi-chang¹; 2; HU Fu-chu²; HUANG Jian-feng¹; HU Gui-bing²; YANG Zhuan-ying²; XIAO Jing²

1.Institute of Tropical Crops Genetic Resources,Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China，Danzhou，Hainan 571737；

2.College of Horticulture，South China Agricultural University，Guangzhou，Guangdong 510642

Keywords:

Litchi chinensis Sonn.; ANS; gene cloning; sequence analysis

PACS:

S 667.1

DOI:

-

Abstract:

Taking the peel and young leaves of Litechi chinensis ‘Nuomici’ at three different stages as material,its ANS gene was cloned and analyzed.The results showed that the anthocyanidin synthase (ANS) was a key enzyme in the anthocyanin biosynthesis.A ANS gene was cloned from litchi pericarp by homologous cloning method.The open reading frame was 1 074 bp，and encoding 357 amino acids.The 1 279 bp length fragment was amplified from genome and analysis，which contained five introns，respectively，in 504~708 bp.It was found that the gene encoding for the protein had close relationship with mountain grape (Vitis vinifera)，cocoa (Theobroma cacao)，citrus (Citrus sinensis)，and other fruit trees through phylogenetic analysis.

References:

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