The seedling adaptaion and transplanting technology of mint vitrification and the influence factors were studied, and a series of management techniques were summarized. The best state and transplanting techniques of mint seedling adaptation were introduced, including substrate choosing, planting methods and protection after transplanting. The results showed that normal roots after 10 days rooting culture+3 days+seedling adaptation 3 days, transplanted into substrate perlite∶sand=2∶1, managed in accordance with the principles of gradual control of water, lightning, humidity, would esaily experience the seedling adaptation, and survival rate of transplant would more than 90%.