|Table of Contents|

Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年18期
Page:
135-139
Research Field:
Publishing date:

Info

Title:
Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method
Author(s):
WANG Jin-luQIAN Tai-linZHAO Mei-ai
Qingdao Key Lab of Germplasm Innovation and Application of Major Crops,College of Life Science,Qingdao Agricultural University,Qingdao,Shandong 266109
Keywords:
Lilium tsingtauense GilgLiliaCeaeshoot tipsencapsulation-vitrificationcryopreservation
PACS:
S 682.2+9
DOI:
-
Abstract:
Taking shoot tips(2~3 mm) of Liliumtsingtauense GilgLiliaCeae.from 28 days cold-hardened bulblets at 4℃ as materials,effects of different explants,sucrose concentration of pre-culture,duration of pre-culture,different encapsulation pretreatment,duration of treatment with PVS2 were studied.The results showed that the most appropriate sucrose concentration was 0.4 mol/L during pre-incubation and the best pre-culture time was 3 d.Shoot-tips were encapsulated in 3% sodium alginate including 0.4 mol/L sucrose and 2 mol/L glycerol after pre-incubation,and PVS2 for 60 min at 0℃,plunged directly into liquid nitrogen for 1 h,then thawed for 2 min at 38℃and washed.At last,the shoot tips were cultured on recovery medium.The highest survival rate was 67.5 % with TTC staining method.

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Last Update: 2014-08-30