|Table of Contents|

OptimizationofAgrobacterium-MediatedTransformationofHBsAgGeneintoCherryTomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年11期
Page:
105-109
Research Field:
Publishing date:

Info

Title:
OptimizationofAgrobacterium-MediatedTransformationofHBsAgGeneintoCherryTomato
Author(s):
ZHANGYan-ting1; GUOBin1; GUANZheng-jun2; DAIJia-kun 3; 4; WEIYa-hui1
1.KeyLaboratoryofResourceBiologyandBiotechnologyinWesternChina，CollegeofLifeScience，NorthwestUniversity，Xi’an,Shaanxi710069；
2.DepartmentofLifeScience，YunchengUniversity,Yuncheng,Shanxi044000；3.EnzymeEngineeringInstituteofShaanxiAcademyofSciences，Xi’an,Shaanxi710600；4.EnzymeEngineeringTechnologyCenterofShaanxi,Xi’an,Shaanxi710600
Keywords:
HBsAggene; cherrytomato; optimization
PACS:
S641.203.6
DOI:
-
Abstract:
Cherrytomatoeswereusedastheexperimentmaterials,andthehighfrequencyregenerationsystemwasestablished.HepatitisBsurfaceantigen（HBsAg）genewasusedtotransformcherrytomatoexplantmediatedbyAgrobacteriumtumefaciens,andtheexplantwasculturedtooptimizethetransformationsystem.Theresultsshowedthat，thehighesttransformationefficiencywasobtainedwhen11doldcotyledonexplantswerepreconditionedontheculturemediumofMS+6-BA3.0mg/L+IAA0.2mg/Lfor2days，infectedwithAgrobacteriumtumefaciens(OD600=0.6)for15min.

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Last Update: 2014-10-09