Study on the Tissue Culture of Dendrobium aphyllum（Roxb.) C.E.C.Fisch(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Tissue Culture of Dendrobium aphyllum（Roxb.) C.E.C.Fisch

Author(s):: DU Gang; LAI Tian-chao; YANG Hai-ying; Key Laboratory of Ethnic Medicine Resource Chemistry,State Ethnic Affairs Commission and Ministry of Education,College of Chemistry and Biotechnology,Yunnan University of Nationalities,Kunming,Yunnan 650500

Keywords:: Dendrobium aphyllum（Roxb.) C.E.C.Fisch; tissue culture; seedling; medium

Abstract:: Taking mature capsule of Dendrobium aphyllum（Roxb.) C.E.C.Fisch as explant,the effect of different medium and phytohormones on seeds germination and root induction of seedlings of Dendrobium aphyllum（Roxb.) C.E.C.Fisch.were studied.The results showed that seedlings could be induced from seed.The most appropriate medium for seedling inducing was MS+NAA 0.5 mg/L+6BA 2.0 mg/L+GA 1.0 mg/L+banana juice 150 g/L+sugar 20 g/L+agar 8 g/L+activated carbon 2 g/L.The most appropriate rooting medium was 1/4MS+NAA 2.0 mg/L+6-BA 0.5 mg/L+banana juice 150 g/L+sugar 20 g/L+agar 8 g/L+activated carbon 2 g/L.

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Last Update: 2014-08-31