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Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年08期
Page:
133-137
Research Field:
Publishing date:

Info

Title:
Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea
Author(s):
ZHANG Wei-ying; YU Li-jie
Higher Education Key Laboratory of Plant biology，College of Life Sciences and Technology，Harbin Normal University,Harbin,Heilongjiang 150025
Keywords:
cabbage; interleukin-4; molecule detection; field survey
PACS:
S 635.603.6
DOI:
-
Abstract:
The il-4 gene was transformed into Brassica oleracea cv.‘Zhonggan 11’ by using Agrobacterium-mediated method.The obtained T3 and T4 generation materials were detected by using PCR amplification，PCR-southern blotting，protein concentration determination and ELISA.Meanwhile the genetic rule of il-4 gene and the phenotypes of progenies were investigated.The results showed that a few positive transgenic cabbage plants were detected in T3 and T4 generation.The genetic rule of il-4 gene in transgenic cabbages didn’t according with Mendel law.Meanwhile，the field survey showed that the disease resistance，height，seed setting rate，growth potential decreased comparing to the control.

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Last Update: 2014-08-31