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Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulaeformis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
126-129
Research Field:
Publishing date:

Info

Title:
Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulaeformis
Author(s):
MA Li-yuan1; ZHANG Ying1; SHANG Fu-qiang1; 2; YAO Rong-sheng2; CUI Jian-guo1
1.College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866；
2.Liaoning Institute of Forest Management，Dandong，Liaoning 118002
Keywords:
Pinus tabulaeformis; embryogenic calluses; immature zygotic embryo; somatic embryogenesis
PACS:
S 791.254
DOI:
-
Abstract:
Effects of different developmental stages of immature zygotic embryo of Pinus tabulaeformis and different combination of plant growth regulators on induction of emvryogenic calluses were studied.The results showed that the optimal time of sampling the immature zygotic embryo for induction of embryogenic calluses in Shenyang region of China was around 10th of July；and when DCR was adopted as the basal medium，combination of low concentration of KT and high concentration of 2,4-D was favorable the induction of embryogenic calluses.The highest initiation rate was up to 88.3% on DCR medium when 2 mg/L of 2,4-D and 0.5 mg/L of KT were added.

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Last Update: 2014-09-03