|Table of Contents|

Optimizing Study on Tissue Technology of the Purple Skin Dendrobe(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年05期
Page:
139-142
Research Field:
Publishing date:

Info

Title:
Optimizing Study on Tissue Technology of the Purple Skin Dendrobe
Author(s):
QI Yong-qiong1; CHEN Zhi-yuan1; LI Kai-yun2; LI Chang-ke1
1.Yunnan Vocational and Technical College of Agriculture，Kunming，Yunnan 650031;
2.West Mountain Forest Station of Kunming，Kunming，Yunnan 650100
Keywords:
purple skin Dendrobe; rapid propagation; seed; agar
PACS:
S 682.31
DOI:
-
Abstract:
With the purple skin Dendrobe seed and stem section as explants，the bud seedling proliferation,rooting,and optimized conditions of plantlets were studied.The results showed that the seed was most appropriate as rapid propagation explant,seeds through the small bud seedling，the proliferation and seedling rooting of the way into complete plant regeneration；And a suitable culture medium formula was explored:beginning the generation of inductive medium for 1/2MS+1 mg/L BA+0.5 mg/L NAA，seed germination directly grow，growth of large green and sterile small bud seedling；Proliferation medium for MS+2 mg/L BA+0.5 mg/L NAA+5% potatoes juice+5% mashed bananas，bud seedling multiple of 4.6 times as high proliferation and growth better，the bud seedling stout；Rooting medium 1/2MS+0.5 mg/L NAA+10% mashed bananas+0.1% activated carbon，take root seedlings root number and root for average to best，root system developed，seedling height，robust，and the leaves deep green.Medium suitable for the AGAR concentration of 6 g/L，therefore，higher multiples，erect plant，leaf also didn’t bend.

References:

[1]冉懋熊,刘家保.云南龙陵紫皮石斛产业发展的思考与建议[J].中国现代中药,2010,12(2):11-13.
[2]刘会清,张爱香,常美花,等.紫皮石斛兰组织培养体系的建立[J].北方园艺,2007(10):192-193.
[3]包雪声,顺庆生,陈立钻.中国药用石斛彩色图谱[M].上海:上海医科大学出版社,复旦大学出版社，2001.
[4]傅玉兰,谷凤,胡传,等.霍山石斛组培快繁技术研究[J].安徽农业科学,2004,32(3)：522-523.
[5]高培元,陈健妙,甘铨.金钗石斛的茎段组织培养与植株再生[J].中草药,2002,33(11):1031-1033.
[6]孙丹,朴炫春,郑艳艳,等.铁皮石斛圆球茎增殖影响因素的研究[J].安徽农业科学,2010,38(32):18150-18151. [7]蓝玉甜,刘世勇,罗玉婷,等.鼓槌石斛种子萌发培养与小苗组培快繁技术研究[J].安徽农业科学,2011,39(9):5380-5382.
[8]卢文芸,唐金刚,乙引,等.五种药用石斛快速繁殖的研究[J].种子,2005,24(5):23-26.
[9]詹忠根.铁皮石斛根尖诱导丛生芽研究[J].中草药,2006,37(6):929-931.
[10]陈文,王延春,刘国民,等.鼓槌石斛未成熟种子无菌萌发与小苗组培快繁的研究[J].贵州科学,2008,26(1):45-51.

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Last Update: 2014-08-27