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Study on Rapid Propagationin vitro of  Mukdenia rossii（Oliv.）Koidz(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年05期
Page:
135-136
Research Field:
Publishing date:

Info

Title:
Study on Rapid Propagationin vitro of  Mukdenia rossii（Oliv.）Koidz
Author(s):
WANG Ke-feng; ZHAO Chun-li; CHEN Li-fei; ZHAO He-xiang; DONG Ran; GU De-feng
College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118
Keywords:
Mukdenia rossii（Oliv.）Koidz; seedling; tissue culture
PACS:
S 681.903.6
DOI:
-
Abstract:
The experiment was based on MS culture media and Mukdenia rossii（Oliv.）Koidz.Used explants were from seedlings of the germination seeds in vitro.An appropriate culture medium was obtained via regulating the concentrations of cytokinin and auxin.The optimal sterilization method for seeds Mukdenia rossii（Oliv.）Koidz was 1% HgCl2 6 min.The propagation coefficient of 5.1 times indicated that MS+6-BA 0.1 mg/ L+NAA 0.1 mg/ L was the optimal culture medium for subculture proliferation.The best rooting medium was 1/2MS.The survival rate of the plantlets was shown to be up to 97%.

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Last Update: 2014-08-27