College of Horticulture and Landscape Architecture, Southwest University,Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education,Key Laboratory of Olericulture,Chongqing 400715
Pleurotus ostreatus was used as material in this study to test activity of laccase, guaiacol oxidase, pyrocatechol oxidase, amylase and carboxymethyl cellulase with medium of various organic nitrogen content in primordia stage, morula stage and coral period. The results showed that formula 5 (Sawdust 97.70%, urea 3%, calcium superphosphate 1%, glucose 1%) was the best option with maximum exocellular enzymatic activity and fastest differentiation. A suitable content of organic nitrogen was good for promoting enzymatic activity during primordium formation of Pleurotus ostreatus, and speeded up the process of primordium formation.