The mycelia growth speed and industrial fruiting process of Pleurotus nebrodensis grown on six culture media were compared. The results showed that on different culture medium, the mycelia growth speed, growth potential, shape of fruit body and biology efficiency of P. nebrodensis were discrepancy. On the fifth culture medium, mycelia of P. nebrodensis grow fast, the average growth speed was 4.19 mm/d, but the total production was low, the average biology efficiency was only 27.30%; on the third culture medium, the mycelia grow not so fast, but the total production was as high as 36.47%, the rate of Class A mushroom arrived 88.74%. In conclusion, the third culture medium (cotton seed hulls, 47%; corn cob, 29%; bran, 16%; corn flour, 6%, lime, 1%; plaster, 1%) was optimal medium for the industrial cultivation of P. nebrodensis.