[1]申瑞楠,张晓申,左红娟,等.金银花WRKY转录因子家族响应白粉病的转录组分析[J].北方园艺,2026,(19):97-105.[doi:10.11937/bfyy.20260410]
　SHEN Ruinan,ZHANG Xiaoshen,ZUO Hongjuan,et al.Transcriptome Analysis of WRKY Transcription Factor Family in Lonicera japonica in Response to Powdery Mildew[J].Northern Horticulture,2026,(19):97-105.[doi:10.11937/bfyy.20260410]
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金银花WRKY转录因子家族响应白粉病的转录组分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年19 页码: 97-105 栏目: 出版日期: 2026-10-15
Title:
Transcriptome Analysis of WRKY Transcription Factor Family in Lonicera japonica in Response to Powdery Mildew
文章编号:
1001-0009(2026)19-0097-09
作者:
申瑞楠; 张晓申; 左红娟; 曹辉; 田芳; 申潇潇
（郑州市农业科技研究院，河南 郑州 450005）
Author(s):
SHEN Ruinan; ZHANG Xiaoshen; ZUO Hongjuan; CAO Hui; TIAN Fang; SHEN Xiaoxiao
(Zhengzhou Institute of Agricultural and Forestry Sciences,Zhengzhou,Henan 450005)
关键词:
金银花; WRKY转录因子; 白粉病; 转录组测序; 生物信息学分析
Keywords:
Lonicera japonica; WRKY transcription factor; powdery mildew; transcriptome sequencing; bioinformatics analysis
分类号:
S567.19
DOI:
10.11937/bfyy.20260410
文献标志码:
A
摘要:
以金银花‘郑银花3号’为试材，对由忍冬叉丝壳菌引起的白粉病感病叶片和健康叶片进行转录组测序分析,并对转录因子WRKY进行筛选与鉴定，利用生物信息学方法对其蛋白理化性质、序列比对、进化关系以及表达模式进行分析，以期为探索金银花WRKY转录因子抗白粉病的调控机制提供参考依据。结果表明：金银花基因组中鉴定得到32个WRKY家族成员，编码氨基酸的数目在164~748 aa，蛋白分子量在17 683.53~80 967.23 Da，LjWRKY蛋白的理论等电点的范围为4.96~9.71，其中有18个蛋白呈酸性，14个蛋白呈碱性，且均为亲水性蛋白。根据基序分析发现，出现频率最高的是Motif 1和Motif 2，推测其是金银花WRKY中的保守基序。在聚类分析中，多个LjWRKY与ATWRKY聚为一类，因而可以推测其具有相似的生物学功能。在WRKY家族的表达分析中，发现有11个基因在DL中的表达量发生了明显上调，其中Lj3C833T11、Lj7A731T27和Lj5C246T14的表达差异最大，这些WRKY基因可能参与了金银花响应白粉病的过程。
Abstract:
Taking Lonicera japonica ‘Zhengyinhua No.3’ as the experimental material,transcriptome sequencing was performed on leaves infected with powdery mildew caused by Microsphaera lonicerae and healthy leaves,followed by the screening and identification of WRKY transcription factors.Bioinformatics methods were subsequently employed to analyze the physicochemical properties of their proteins,sequence alignment,evolutionary relationships,and expression patterns,in order to provide a reference for investigating the regulatory mechanisms of WRKY transcription factors in Lonicera japonica against powdery mildew.The results showed that 32 members of the WRKY family were identified in the Lonicera japonica genome,encoding proteins with amino acid numbers ranging from 164 to 748 aa and molecular weights from 17 683.53 Da to 80 967.23 Da.The theoretical isoelectric points of LjWRKY proteins ranged from 4.96 to 9.71,among which 18 proteins were acidic and 14 were basic,all of which were hydrophilic proteins.Motif analysis revealed that Motif 1 and Motif 2 had the highest occurrence frequencies,suggesting that they were conserved motifs in Lonicera japonica WRKY proteins.Phylogenetic clustering analysis showed that multiple LjWRKY proteins were clustered with ATWRKY proteins,implying that they may possess similar biological functions.Expression analysis of the WRKY family indicated that the expression levels of 11 genes were significantly up-regulated in powdery mildew-infected leaves (DL),with Lj3C833T11,Lj7A731T27 and Lj5C246T14 exhibiting the most significant differential expression.These WRKY genes may be involved in the response of Lonicera japonica to powdery mildew infection.

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备注/Memo

第一作者简介：申瑞楠（1995-），女，硕士，研究实习员，现主要从事甘中药材新品种选育及栽培技术等研究工作。E-mail：389960601@qq.com.责任作者：张晓申（1976-），男,博士，副研究员，现主要从事中药材新品种选育及栽培技术等研究工作。E-mail:nkszyc@126.com.基金项目：河南省科技兴林资助项目(豫财环资［2025］44号)。收稿日期：2026-01-30

更新日期/Last Update: 2026-10-19