ZHOU Hailin,CHEN Mengfan,MAO Renjun,et al.Analysis of Differentially Expressed Genes Caused by High-altitude Mutagenesis in Wild Ziziphus jujuba From Northern Shaanxi[J].Northern Horticulture,2026,(12):30-36.[doi:10.11937/bfyy.20254322]
陕北野生圆酸枣高空诱变差异基因分析
- Title:
- Analysis of Differentially Expressed Genes Caused by High-altitude Mutagenesis in Wild Ziziphus jujuba From Northern Shaanxi
- 文章编号:
- 1001-0009(2026)12-0030-07
- 分类号:
- S665.1
- 文献标志码:
- A
- 摘要:
- 以陕北野生圆酸枣种仁为试材，在搭载DEAR-2K航天器进行高空诱变处理后，对返回种仁及对照采用无菌发芽和组培扩繁方法获得组培苗，同时采用IlluminaTM平台对其进行转录组测序，结合生物信息学方法筛选差异表达基因，并进行GO功能与KEGG通路富集分析，研究了高空诱变对酸枣的变异效应，以期为酸枣高空诱变育种提供参考和早期鉴定依据。结果表明：酸枣经高空诱变处理后鉴定到2 544个差异表达基因，其中1 031个基因表达上调，1 513个基因表达下调。GO与KEGG富集分析一致显示，这些基因主要涉及两大类核心通路,一是细胞增殖与遗传信息相关通路，如DNA复制、细胞周期调控、同源重组；二是次生代谢相关通路，如苯丙烷合成、类黄酮生物合成与降解。综上，高空诱变效应在多代培养后依然稳定存在，系统影响了酸枣细胞增殖与药用成分代谢两大网络，证明高空诱变是获得具有潜在矮化、高药用成分酸枣新种质的有效途径。高空诱变处理与转录组分析相结合可辅助早期鉴定、提高选育效率。
- Abstract:
- Taking wild round Z.jujuba seeds from northern Shaanxi as test materials,after high-altitude mutagenesis treatment via the DEAR-2K spacecraft,the retrieved seeds and controls were subjected to sterile germination and tissue culture propagation to obtain plantlets.Transcriptome sequencing of the plantlets was performed using the IlluminaTM platform.Differentially expressed genes were screened using bioinformatics methods,followed by GO function and KEGG pathway enrichment analysis.The mutagenic effects of high-altitude mutagenesis on Z.jujuba were investigated,in order to provide references and early identification criteria for high-altitude mutagenesis breeding of Z.jujuba.The results showed that 2 544 differentially expressed genes were identified after high-altitude mutagenesis,among which 1 031 were up-regulated and 1 513 were down-regulated.Both GO and KEGG enrichment analyses consistently revealed that these genes were primarily involved in two major categories of core pathways.Firstly,pathways related to cell proliferation and genetic information,such as DNA replication,cell cycle regulation,and homologous recombination.Secondly,pathways associated with secondary metabolism,included phenylpropanoid biosynthesis,flavonoid biosynthesis,and degradation.The study concluded that the effects of high-altitude mutagenesis remain stable across multiple generations of cultivation and systematically influence two key networks in Z.jujuba-cell proliferation and medicinal component metabolism.This demonstrated that high-altitude mutagenesis was an effective approach for developing new germplasms of Z.jujuba with potential dwarfing traits and enhanced medicinal composition.Combining high-altitude mutagenesis with transcriptome analysis can aid in early-stage identification and improve breeding efficiency.
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备注/Memo
第一作者简介：周海霖（2000-），男，硕士研究生，研究方向为植物遗传学。E-mail：1020681195@qq.com.责任作者：栗现芳（1981-），女，博士，副教授，现主要从事植物遗传多样性和分子育种等研究工作。E-mail：lixianfang810702@163.com.基金项目：陕西省自然科学基础研究计划资助项目（2025JC-YBMS-236）；延安天宇科佑科技有限公司横向资助项目（206021235）；延安合源生物科技有限公司横向资助 项目（206020872）；延安市科技计划资助项目（2022SLJBZ-012）；国家自然科学基金资助项目（31860535）。收稿日期：2025-12-16