WANG Zhenze,LU Minghui,LI Dawei.Research Progress on Microspore Culture of Pepper (Capsicum annuum L.)[J].Northern Horticulture,2025,(9):131-137.[doi:10.11937/bfyy.20244336]
辣椒小孢子培养研究进展
- Title:
- Research Progress on Microspore Culture of Pepper (Capsicum annuum L.)
- 文章编号:
- 1001-0009(2025)09-0131-07
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 小孢子培养是获取单倍体的重要技术途径之一，其技术过程复杂、操作难度大，并且在辣椒上存在基因型依赖、胚诱导率差、子叶胚产量低、幼胚成苗困难等问题，目前在辣椒育种工作中还难以大规模应用。为了进一步优化和完善辣椒小孢子培养技术体系，推动其在辣椒新品种选育中的应用，该文综述了近年来有关小孢子胚胎发生的理论基础进展，以及辣椒小孢子培养技术的研究探索成果，重点总结了基因型、生长环境、预处理条件、培养基及其附加物、单倍体植株加倍及倍性鉴定方式等对辣椒小孢子培养的影响，以期为发展和优化辣椒小孢子培养体系、加快构建辣椒双单倍体群体、高效选育优良辣椒品系提供参考依据。
- Abstract:
- Microspore culture is one of the important techniques for the generation of plant haploids，however，its operation is complex and difficult，and there are problems for its application in pepper，such as genotype-dependence，lower embryo-induction rate，lower cotyledonary embryo yields，and difficulties in the growth of seedlings developed from embryos，which made it difficult to be used in pepper breeding with a large scale at present.To further optimize and improve the technology system of pepper microspore culture，and promote its application in pepper breeding，this paper reviewed recent advancements in the theoretical foundations of microspore embryogenesis and the achievements of research on pepper microspore culture techniques.The study focused on the effects of genotype，growth environment，pretreatment conditions，culture medium and additives during pepper microspore culture，the chromosome-doubling of haploid plant，and the identification of ploidy,in order to provide a reference for advancing the development and optimization of pepper microspore culture systems，accelerating the establishment of pepper double-haploid populations，and efficiently generating pepper elite lines.
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备注/Memo
第一作者简介：王振泽（2000-），男，硕士研究生，研究方向为园艺农艺与种业。E-mail:wzz1420030479@163.com.责任作者：李大伟（1979-），男，博士，副教授，现主要从事园艺作物基因编辑和蔬菜分子设计育种与分子生物学等研究工作。E-mail：xndavid@nwsuaf.edu.cn.基金项目：陕西省科技计划资助项目（K3030223018）；国家大宗蔬菜产业技术体系专项资助项目（CARS-23-G22）；陕西省农业核心技术攻关资助项目（2024NYGG008-2）。收稿日期：2024-11-04