PEI Xiangli,HAN Ce,LIU Hongfei,et al.Physiological and Biochemical Responses of Jujube Under Different Salt Stress and Comprehensive Evaluation[J].Northern Horticulture,2025,(5):39-47.[doi:10.11937/bfyy.20243488]
不同盐胁迫下冬枣生理生化响应及综合评价
- Title:
- Physiological and Biochemical Responses of Jujube Under Different Salt Stress and Comprehensive Evaluation
- 文章编号:
- 1001-0009(2025)05-0039-09
- Keywords:
- jujube; brackish water irrigation; physiological and biochemical response; principal component analysis
- 分类号:
- S 665.1
- 文献标志码:
- A
- 摘要:
- 以冬枣为试材，采用大田试验的方法，研究了不同生育期内5种矿化度（1、2、3、4 g·L-1和5 g·L-1）的微咸水灌溉对冬枣叶片相对叶绿素、脯氨酸、丙二醛、内源激素含量和叶片保护酶活性的影响，并采用主成分分析法识别了表征冬枣耐盐性的生理生化指标，以期为探索冬枣在不同矿化度微咸水灌溉下的生理生化响应、揭示其对盐胁迫的适应机制提供参考依据。结果表明：随着灌水矿化度的增加和生育期的推进，相对叶绿素、脱落酸含量呈先升高后降低的趋势，脯氨酸含量、丙二醛含量总体上呈上升趋势，而赤霉素、吲哚乙酸含量则呈降低趋势；相同矿化度下，超氧化物歧化酶、过氧化物酶、过氧化氢酶活性随生育期的推进而升高；在不同生育期，赤霉素、吲哚乙酸含量以及超氧化物歧化酶、过氧化物酶、过氧化氢酶活性随灌水矿化度的增加表现出不同的变化趋势。冬枣对盐胁迫的生理生化响应综合评价中，通过主成分分析提取2个主成分（5项指标）可作为冬枣耐盐性综合评价指标。在微咸水矿化度不超过3 g·L-1，冬枣可以通过增加自身脯氨酸和脱落酸含量、提高叶片保护酶活性以及降低赤霉素和吲哚乙酸含量来抵御盐胁迫，展现出一定的耐盐性，但超过3 g·L-1，冬枣酶活性受到抑制，激素合成受阻，进而影响体内调控机制；脯氨酸、丙二醛含量以及超氧化物歧化酶、过氧化物酶和过氧化氢酶活性可以作为冬枣耐盐性评价的关键指标。
- Abstract:
- Taking jujube as the test material，the effects of five mineralization levels (1,2,3,4 g·L-1 and 5 g·L-1) of brackish water irrigation on the relative chlorophyll content，proline content，malondialdehyde content，endogenous hormone content and leaf protective enzyme activity of jujube leaves were studied in a large-scale field experiment，and the principal component analysis was used to identify physiological and biochemical indexes that characterize the salt tolerance of jujube.The main component analysis was used to identify the physiological and biochemical indexes that characterize the salinity tolerance of jujube，in order to provide reference for exploring the physiological and biochemical responses of jujube under irrigation with brackish water of different mineralization degrees，and revealing its adaptive mechanism to salt stress.The results showed that with the increase of irrigation water mineralization and the advancement of fertility period，the relative chlorophyll and abscisic acid content showed a trend of increasing and then decreasing，the proline content and malondialdehyde content showed a general trend of increasing，while the content of gibberellin and indole acetic acid showed a trend of decreasing；under the same mineralization，the activities of superoxide dismutase，peroxidase，catalase and hydrogen peroxide dismutase increased with the advancement of the fertility period；at different fertility periods，the activities of the contents of gibberellin and indoleacetic acid as well as the activities of superoxide dismutase，peroxidase and catalase showed different trends with the increase of irrigation water mineralization.In the comprehensive evaluation of physiological and biochemical responses of jujube to salt stress，2 principal components (5 indexes) were extracted by principal component analysis，which could be used as the comprehensive evaluation indexes of salinity tolerance of jujube.In brackish water mineralization not more than 3 g·L-1，jujube could resist salt stress by increasing its own proline and abscisic acid content，improving leaf protective enzyme activity，and decreasing gibberellin and indoleacetic acid content，showing a certain degree of salinity tolerance；however，more than 3 g·L-1，jujube enzyme activity was inhibited，hormone synthesis was blocked，which in turn affected the regulatory mechanismin the body；proline，malondialdehyde content，and proline，malondialdehyde content and the activities of superoxide dismutase，peroxidase and catalase could be used as the key indexes for the evaluation of salt tolerance in jujube.
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备注/Memo
第一作者简介：裴向丽（2001-），女，硕士研究生,研究方向为农业水资源高效利用。E-mail：pxl662791@163.com.责任作者：徐征和（1968-），男，博士，教授,现主要从事农业水资源高效利用等研究工作。E-mail：stu_xuzh@ujn.edu.cn.基金项目：山东省自然科学基金资助项目(ZR2020ME253);山东省重点研发计划（重大科技创新工程）资助项目(2024CXGC010912)；济南大学优秀博士资助项目(XBS2461)；济南大学2023年学科交叉会聚建设资助项目（XKJC-202305）。收稿日期：2024-09-01