SONG Ying,LIU Na,LYU Litao,et al.Murtagenic Effect and Indentification Analysis of 60Co-γ Prudiation on Stropharia rugosoannulata[J].Northern Horticulture,2025,(5):9-15.[doi:10.11937/bfyy.20243377]
60Co-γ射线对大球盖菇的诱变效应及鉴定分析
- Title:
- Murtagenic Effect and Indentification Analysis of 60Co-γ Prudiation on Stropharia rugosoannulata
- 文章编号:
- 1001-0009(2025)05-0009-07
- Keywords:
- Stropharia rugosoannulata; mutagenesis; biological characteristics; nutritional components
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以大球盖菇菌株‘FQ’为试材，采用60Co-γ射线对菌株多孢悬液进行辐照诱变的育种方法，研究白色诱变杂交子的真实性、出菇特性、菌丝生物学特性及不同营养成分对菌丝生长速度的影响，以期为选育白色大球盖菇新菌株、丰富大球盖菇种质资源、新品种选育提供参考依据。结果表明：‘辽白球盖菇1号’为大球盖菇白色品种的首次报道。栽培试验表明，该菌株菌盖栽培性状稳定，生物转化率89%。该菌株菌丝生长适宜温度为25 ℃；最适碳源为麦芽糖，最适氮源为大豆蛋白胨，最适无机盐为磷酸二氢钾。子实体营养成分分析表明，该菌株营养丰富，氨基酸组成较优，钙含量为出发菌株‘FQ’的2倍。
- Abstract:
- Taking stain ‘FQ’ of Stropharia rugosoannulata as the test material，the breeding method of irradiation mutagenesis of the strain′s multiple spore suspension with 60Co-γ rays was used to study the authenticity,fruiting characteristics,mycelial biological characteristics of white mutant hybrids,and the effect on the growth rate of mycelium with different nutritional components,in order to provide reference for breeding new strains,enriching the germplasm resources of Stropharia rugosannulata and selecting new varieties.The results showed that ‘LiaoXQ01’ was the first report of a white variety of Stropharia rugosannulata.Demonstration cultivation showed that strain ‘LiaoXQ01’ was stable in cultivation characteristics，with a biological efficiency of 89%.The optimal temperature for mycelial growth of ‘LiaoXQ01’ was 25 ℃；the optimal carbon source was maltose，the optimal nitrogen source was soy peptone，and the most suitable inorganic salt was KH2PO4.The analysis of the nutritional components showed that ‘LiaoXQ01’ was nutritious，with an excellent amino acid composition and the calcium content was twice that of the starting strain ‘FQ’.
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备注/Memo
第一作者简介：宋莹（1982-），女，硕士，研究员，现主要从事食用菌种质资源评价与创新利用等研究工作。E-mail:sy.512@163.com.责任作者：张敏（1972-），女，博士，研究员，现主要从事食用菌育种与栽培技术等研究工作。E-mail:772579543@qq.com.基金项目：现代农业产业技术体系资助项目（CARS-20）。收稿日期：2024-08-22