LING Na,HAN Lingyun,HUANG Zhijia,et al.Effects of MeJA on Seed Germination and Seedling Growth of Glebionis coronaria Under Salt Stress[J].Northern Horticulture,2024,(24):8-14.[doi:10.11937/bfyy.20242248]
茉莉酸甲酯对盐胁迫下茼蒿种子萌发及幼苗抗性的影响
- Title:
- Effects of MeJA on Seed Germination and Seedling Growth of Glebionis coronaria Under Salt Stress
- 文章编号:
- 1001-0009(2024)24-0008-07
- 分类号:
- S 636.9
- 文献标志码:
- A
- 摘要:
- 以小叶茼蒿种子为试材,采用培养皿萌发水培法,研究了不同施用浓度(0.02、0.20、2.0、20、200 μmol·L-1)和浸泡时间(0、3、6、9、12、15 h)的外源茉莉酸甲酯(MeJA)对盐胁迫下茼蒿种子萌发指标(发芽率、发芽指数、发芽时间和芽长)、幼苗生长指标(根长、茎长、相对含水量)和幼苗生理指标(叶绿素、可溶性糖、可溶性蛋白质、脯氨酸含量)的影响,以期为茉莉酸甲酯在茼蒿种子耐盐萌发及幼苗栽培管理中的合理使用提供参考依据。结果表明:150 mmol·L-1NaCl胁迫下,MeJA处理种子均可以不同程度缓解盐害,其中20 μmol·L-1MeJA溶液处理6 h为最佳处理浓度和时间,显著提高茼蒿种子的发芽率和发芽指数,而且对茼蒿幼苗生长的效果也较好,叶绿素含量及渗透调节物质含量增加。
- Abstract:
- Taking the seeds of Glebionis coronaria as the test material,the effects of exogenous methyl jasmonate (MeJA) at different application concentrations (0.02,0.20,2.0,20,200 μmol·L-1) and soaking times (0,3,6,9,12,15 hours) on the germination indicators (germination rate,germination index,germination time,and shoot length),seedling growth indicators (root length,stem length,relative water content),and physiological indicators (chlorophyll,soluble sugar,soluble protein,proline content) of Glebionis coronaria seeds under salt stress were studied by using a water culture method with small leaves,in order to explore the optimal application concentration and treatment time of MeJA to alleviate salt damage in Glebionis coronaria.The results showed that under 150 mmol·L-1 NaCl stress,MeJA-treated seeds could alleviate salt damage to varying degrees.Among them,treatment with a 20 μmol·L-1 MeJA solution for 6 hours was the optimal concentration and time,significantly increasing seed germination rate and germination index,and also showed good effects on the growth of Glebionis coronaria seedlings.The content of chlorophyll and osmotic regulating substances increased.
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备注/Memo
第一作者简介:凌娜(1986-),女,硕士,副教授,现主要从事植物遗传育种等研究工作。E-mail:lingnas1985@163.com.责任作者:孟凡荣(1973-),女,博士,教授,现主要从事植物表观遗传学等研究工作。E-mail:mengfanrong73@yahoo.com.cn.基金项目:河南省高等学校青年骨干教师培养计划资助项目(2023GGJS188);河南省科技攻关资助项目(242102112208)。收稿日期:2024-05-28