MA Huilin,TIAN Ziye,LIN Shusen,et al.Comparative on Phenotypic Diversity of Cypripedium ventricosum Sw.With Different Flower Colors in Changbai Mountain Area[J].Northern Horticulture,2024,(21):44-51.[doi:10.11937/bfyy.20241756]
长白山区不同花色大花杓兰的表型多样性比较
- Title:
- Comparative on Phenotypic Diversity of Cypripedium ventricosum Sw.With Different Flower Colors in Changbai Mountain Area
- 文章编号:
- 1001-0009(2024)21-0044-08
- 分类号:
- S 682.3
- 文献标志码:
- A
- 摘要:
- 以长白山区6种不同花色的野生大花杓兰为试材，采用表型多样性分析、聚类分析及主成分分析等方法，研究其花部颜色及器官形态和根、茎、叶的形态特征，以及不同花色大花杓兰的表型多样性，以期为该地区野生大花杓兰的野生资源保护与分类学研究提供参考依据。结果表明：1）该地区6种不同花色的大花杓兰种群海拔分布范围为381~411 m，坡向为西北（NW）方向，坡度为10°~15°的缓坡。主要的伴生种有58分类群。2）6种不同花色的大花杓兰花部器官颜色间存在较大差异，DH4和DH6的各个花部器官均较集中，各个花部器官的颜色最相似；DH1和DH2的各个花部器官较分散，表明其各个花部器官的颜色差异性较大。3）数量性状遗传多样性的变异系数为7.19%~44.26%，质量性状Shannon-Wiener多样性指数为1.74~1.79；在R型聚类的第一类群分析表明，不同花部器官的同类数量性状和同一花部器官的不同数量性状分布紧密；在主成分分析中以特征值>1.0为3个主成分，其与花部器官相关的8个性状的特征向量绝对值较高，表明这8个与花部器官相关的性状在一定程度上是引起大花杓兰表型变异的主要因素。
- Abstract:
- Taking six different colored wild Cypripedium venturicosum Sw.from Changbai Mountain area as the test materials,phenotypic diversity analysis，cluster analysis and principal component analysis were used to investigate their flower color and organ morphology，as well as the morphological characteristics of roots，stems，and leaves,in order to provide reference for the protection and taxonomic research of wild Cypripedium venturicosum Sw.resources in this area.The results showed that,1) the altitude distribution range of six different colored populations of C.macranthos in this forest area was 381-411 m，with a slope direction of northwest and a gentle slope of 10°-15°.The main associated species had 58 taxa.2) According to the comparative analysis of the color of the flower organs of six different flower colors of the C.macranthos，there were significant differences in the color of their flower organs.The various flower organs of DH4 and DH6 were relatively concentrated，and the colors of each flower organ were the most similar；the scattered floral organs of DH1 and DH2 indicate significant color differences among their respective floral organs.3) The coefficient of variation of genetic diversity for quantitative traits was 7.19%-44.26%，and the Shannon-Wiener diversity index for qualitative traits was 1.74-1.79；in the first group analysis of R-type clustering，it was found that the distribution of quantitative indicators of the same type in different floral organs was closely related，such as sepal length and petal length.Different quantitative characteristics of the same floral organ were also closely clustered，such as flower length and flower width；in principal component analysis，with eigenvalues>1.0 as the three principal components，the absolute values of the eigenvectors of the eight traits related to floral organs were relatively high，indicated that these eight traits related to floral organs were to some extent the main factors causing phenotypic variation in C.macranthos.
参考文献/References:
［1］中国科学院中国植物研究所.中国植物志［M］.北京:科学出版社，2019.［2］李春光，金洙哲.延边植物图鉴［M］.延吉:延边人民出版社，2023.［3］陈心启．中国杓兰属植物［M］．北京:科学出版社,2013．［4］CRIBB P,GREEN P．The genus Cypripedium［M］．Portland,Or．:Timber Press,1997.［5］AVERYANOV L V.The genus Cypripedium (Orchidaceae) in Russia［J］.Lindleyana，2000（15）：197-221.［6］孙明洲.吉林省生物多样性:菌物志植物志动物志(植物志)第七卷［M］.长春:吉林教育出版社，2021.［7］黄树贤总,李德润,徐胜斌.中华人民共和国民政部编．中华人民共和国政区大典-吉林省卷［M］．北京:中国社会出版社,2016．［8］傅沛云,李冀云．东北植物检索表［M］.2版．北京:科学出版社,1995．［9］徐可,王涛,张毓．兰科杓兰属(Cypripedium)植物种子非共生萌发研究进展［J］．生物资源,2020,42(1):43-48.［10］金英花,全雪丽,张美淑,等．长白山自然保护区重点保护植物名录分析研究［J］．种子,2020,39(12):62-67.［11］张毓,张启翔,赵世伟,等．濒危植物大花杓兰胚与珠被发育的研究［J］．园艺学报,2010,37(1):72-76.［12］JIANG H,CHEN M C,LEE Y I．In vitro germination and low-temperature seed storage of Cypripedium lentiginosum P．J．Cribb & S．C．Chen,a rare and endangered lady’s slipper orchid［J］．Scientia Horticulturae,2017,225:471-479.［13］王艳丽．大花杓兰生物学特性与组织培养研究［D］．延吉:延边大学,2009.［14］吕惠子,邱爽,杨威,等．长白山区天桥岭野生杓兰属植物的多样性研究［J］．中国野生植物资源,2012,31(6):72-73,77.［15］李平平,张祥,刘雨婷,等．辣椒63份种质果皮颜色与呈色物质的关系［J］．园艺学报,2022,49(7):1589-1601.［16］崔虎亮,嘉婧,曹如姬．不同萱草品种花色表观特征与类黄酮物质的差异分析［J］．北方园艺,2023(23):68-74.［17］中华人民共和国农业部．植物新品种特异性、一致性和稳定性测试指南 蝴蝶兰:NY/T 2230—2012［S］．北京:中国农业出版社,2013.［18］王钦,黄捷,涂松,等．蝴蝶兰不同品种表型性状遗传多样性分析［J］．西南林业大学学报(自然科学版),2023,43(6):8-18.［19］李艳冬,李运兴,殷益明,等.基于主成分分析蝴蝶兰质量评价模型建立［J］.北方园艺,2024(6):61-68.［20］徐东旭,姜翠棉,宗绪晓．蚕豆种质资源形态标记遗传多样性分析［J］．植物遗传资源学报,2010,11(4):399-406.［21］吕伟,韩俊梅,任果香,等．山西芝麻种质资源遗传多样性分析［J］．作物杂志,2019(5):57-63.［22］火艳,招雪晴,黄厚毅,等．观赏石榴表型遗传多样性分析［J］．浙江农林大学学报,2020,37(5):939-949.［23］陈丽飞,刘淑英,孙叶迎,等．长白山区杓兰属植物的种子微形态特征［J］．东北林业大学学报,2012,40(10):134-136.［24］陈丽飞,孙叶迎,王满玲,等．长白山区杓兰属植物的叶片表皮研究［J］．北方园艺,2012(23):68-70.［25］陈丽飞,赵和祥,顾德峰,等.5种杓兰属植物花粉的微形态扫描电镜观察［J］．江苏农业科学,2013,41(9):161-163.［26］钟海丰,陈剑锋,陈宇华,等．蝴蝶兰主要数量性状DUS测试性能研究［J］．东南园艺,2019,7(6):14-21.［27］宋一岚,张英杰,孙纪霞,等．蝴蝶兰70份资源观赏性状综合评价［J］．热带作物学报,2020,41(1):43-48.［28］肖鑫辉,李开绵,许瑞丽,等．国内外栽培木薯(Manihot esculenta crantz)种质资源表型多样性分析［J］．植物遗传资源学报,2017,18(1):94-105.［29］都真真,李锡香,宋江萍,等.228份引进大蒜资源的表型多样性分析及适应性初步评价［J］．植物遗传资源学报,2019,20(5):1186-1196.
相似文献/References:
[1]张亚琦,王 宁,赵 越,等.青海高原西伯利亚白刺天然居群表型多样性研究[J].北方园艺,2013,37(09):64.
ZHANG Ya-qi,WANG Ning,ZHAO Yue,et al.Phenotypic Diversity for Natural Populations of Nitraria sibirica Pall.in Qinghai Plateau[J].Northern Horticulture,2013,37(21):64.
[2]魏海斌,朱春云,刘小利,等.青海核桃种质资源表型多样性研究[J].北方园艺,2015,39(12):20.[doi:10.11937/bfyy.201512005]
WEI Hai-bin,ZHU Chun-yun,LIU Xiao-li,et al.Research on the Phenotypic Diversity of Walnut Germplasm Resources in Qinghai[J].Northern Horticulture,2015,39(21):20.[doi:10.11937/bfyy.201512005]
[3]李叶芳,马诗雨,宋杰,等.大白花杜鹃三个天然居群的表型多样性分析[J].北方园艺,2019,43(01):115.[doi:10.11937/bfyy.20181582]
LI Yefang,MA Shiyu,SONG Jie,et al.Phenotypic Diversity of Three Natural Populations of Rhododendron decorum[J].Northern Horticulture,2019,43(21):115.[doi:10.11937/bfyy.20181582]
[4]王宁,牛立新,张延龙,等.元宝枫天然居群表型多样性研究[J].北方园艺,2019,43(08):90.[doi:10.11937/bfyy.20183634]
(College of Landscape Architecture and Arts,Northwest A&F University,Yangling,et al.Phenotypic Diversity of Acer truncatum Natural Populations in China[J].Northern Horticulture,2019,43(21):90.[doi:10.11937/bfyy.20183634]
[5]王妍欣,陆彪,管岳,等.伊犁野果林新疆忍冬表型多样性分析[J].北方园艺,2024,(17):8.[doi:10.11937/bfyy.20240824]
WANG Yanxin,LU Biao,GUAN Yue,et al.Phenotypic Diversity Analysis of Lonicera tatarica L.in Yili Wild Fruit Forest[J].Northern Horticulture,2024,(21):8.[doi:10.11937/bfyy.20240824]
备注/Memo
第一作者简介：麻蕙琳（1999-），女，硕士研究生，研究方向为长白山地区野生植物的生态调查与分类学。E-mail：1434966210@qq.com.责任作者：金英花（1982-），女，博士，副教授，现主要从事长白山地区野生植物的生态调查与分类学等研究工作。E-mail：yhuajin@ybu.edu.cn.基金项目：吉林省教育厅“十三五”科学技术资助项目（JJKH20200523KJ)；延边大学青年基金资助项目（201816）。收稿日期：2024-04-23