HAO Shengjie,HUO Jiaxing,LI Yuye,et al.Effects of Silicon and Arbuscular Mycorrhiza on the Roots of Glycyrrhiza uralensis Under Drought Stress[J].Northern Horticulture,2024,(20):99-109.[doi:10.11937/bfyy.20240565]
干旱胁迫下硅和丛枝菌根对甘草根的影响
- Title:
- Effects of Silicon and Arbuscular Mycorrhiza on the Roots of Glycyrrhiza uralensis Under Drought Stress
- 文章编号:
- 1001-0009(2024)20-0099-11
- Keywords:
- drought stress; Glycyrrhiza uralensis; application of Si; arbuscular mycorrhizal fungi; secondary metabolites
- 分类号:
- S 567.7+1
- 文献标志码:
- A
- 摘要:
- 以甘草为试材，采用2个外源硅（Si）浓度水平、2个丛枝菌根真菌（AMF）接种水平和5个不同湿度处理的方法进行随机组合，并对甘草的产量、矿物质吸收、抗氧化潜力和次生代谢产物进行检测评估，研究了干旱胁迫下Si和AMF对甘草根系的影响，以期为甘草产业的可持续发展提供参考依据。结果表明：在干旱胁迫条件下，与对照相比，甘草的根长、直径和干质量（DG）显著降低（P<0.05）。Si和AMF显著（P<0.05）增加了根面积并提高了甘草根系的定殖水平。在G40处理下，Si和AMF的交互作用显著提高了甘草酸（GA）含量（26.62 mg·g-1DG）。在严重干旱条件下，甘草的总黄酮（TF）和酚类物质（TP）含量减少，但Si和AMF显著增加了没食子酸（16.46 mg·g-1DG）和反式阿魏酸（SA）（1.11 mg·g-1DG）的含量。此外，Si和AMF的协同作用对根系中的氮（N）和磷（P）浓度产生了正向影响，分别增加了53.51%和74.07%。在低灌水条件下，根系中的磷和硅浓度显著增加，而在Si处理的植物中，钾（K）浓度在G100、G80和G40处理下也有所增加，幅度为38.5%～44.7%。综上，外源Si和AMF对改善植物生长和增强甘草代谢物积累具有协同作用，有助于减轻干旱胁迫的不利影响，为甘草产业应对干旱问题提供了重要的启示。
- Abstract:
- Taking Glycyrrhiza uralensis as the test material，two levels of exogenous silicon (Si) concentration，two levels of arbuscular mycorrhizal fungi (AMF) inoculation，and five different humidity treatments were randomly combined to detect and evaluate the yield，mineral absorption，antioxidant potential，and secondary metabolites of licorice.The effects of Si and AMF on Glycyrrhiza uralensis root system under drought stress were studied，in order to provide reference for the sustainable development of Glycyrrhiza uralensis industry.The results showed that,under drought stress,the root length,diameter,and dry weight (DG) of licorice significantly decreased compared to control group (P<0.05).However,Si and AMF significantly increased root area (P<0.05) and root colonization levels (P<0.05).Under the G40 treatment,the interaction between Si and AMF significantly increased the glycyrrhizic acid (GA) content (26.62 mg·g-1DG).Under severe drought conditions,total flavonoids (TF) and phenolic compounds (TP) decreased,but the application of Si and AMF significantly increased the levels of gallic acid (16.46 mg·g-1DG) and trans-ferulic acid (SA) (1.11 mg·g-1DG).Furthermore,the synergistic effects of Si and AMF positively impacted nitrogen (N) and phosphorus (P) concentrations in the roots,increasing them by 53.51% and 74.07%,respectively.Under low irrigation levels,the concentrations of phosphorus and silicon in the roots significantly increased,and potassium (K) concentrations also increased under Si treatment in the G100,G80,and G40 treatments,ranging from 38.5% to 44.7%.This study demonstrates that exogenous Si and AMF have a synergistic effect in improving plant growth and enhancing the accumulation of metabolites in licorice,providing important insights for mitigating the adverse effects of drought on the licorice industry.
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备注/Memo
第一作者简介：郝胜杰（2003-），男，本科，助理实验员，现主要从事中药学、生物与医药等研究工作。E-mail：haosj@st.gsau.edu.cn.基金项目：国家中药材产业技术体系建设专项资金资助项目(CARS-21)；甘肃农业大学SIETP资助项目（202301058）。收稿日期：2024-02-02